추천 제품
제품명
Anti-Integrin beta1 Antibody, Cytosolic, serum, Chemicon®
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, rat, pig, human
제조업체/상표
Chemicon®
기술
immunoprecipitation (IP): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ITGB1(3688)
일반 설명
Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
특이성
Reactive with human, mouse and rat integrin β-1, as confirmed by western blot. Not reactive with other integrins.
면역원
Epitope: C-terminus
Synthetic peptide corresponding to the c-terminal cytoplasmic domain of human β-1 integrin.
애플리케이션
Anti-Integrin beta1 Antibody, Cytosolic detects level of Integrin beta1 & has been published & validated for use in IP & WB.
Immunoprecipitation:
1 μL of antiserum per 25 μg total cell protein (in 40 μL). ) 0.5% triton X-100 lysis buffer with proteinase inhibitors is suggested.
Optimal working dilutions must be determined by end user.
1 μL of antiserum per 25 μg total cell protein (in 40 μL). ) 0.5% triton X-100 lysis buffer with proteinase inhibitors is suggested.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
품질
Western Blot Analysis:
1:500 to 1:1000 dilution of this antibody was used for detection of integrin beta1 in whole cell lysate under non-reducing. Size of beta 1 integrin under non-reduced conditions is approximately 110 kDa; often a 220 kDa dimer is observed as well; beta -1 under reduced conditions runs as a 130kDa band with higher molecular weight aggregates as well.
1:500 to 1:1000 dilution of this antibody was used for detection of integrin beta1 in whole cell lysate under non-reducing. Size of beta 1 integrin under non-reduced conditions is approximately 110 kDa; often a 220 kDa dimer is observed as well; beta -1 under reduced conditions runs as a 130kDa band with higher molecular weight aggregates as well.
표적 설명
~130 kDa
물리적 형태
Rabbit polyclonal serum containing no preservatives
Unpurified
저장 및 안정성
Stable for 1 year at -20ºC from date of receipt.
분석 메모
Control
U251 cell line
U251 cell line
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells.
Eva, R; Dassie, E; Caswell, PT; Dick, G; ffrench-Constant, C; Norman, JC; Fawcett, JW
The Journal of Neuroscience null
Evgeny A Zemskov et al.
PloS one, 6(4), e19414-e19414 (2011-05-11)
Although endosomal compartments have been suggested to play a role in unconventional protein secretion, there is scarce experimental evidence for such involvement. Here we report that recycling endosomes are essential for externalization of cytoplasmic secretory protein tissue transglutaminase (tTG). The
Regulation of ectoplasmic specialization dynamics in the seminiferous epithelium by focal adhesion-associated proteins in testosterone-suppressed rat testes.
Wong, CH; Xia, W; Lee, NP; Mruk, DD; Lee, WM; Cheng, CY
Endocrinology null
Iwona Majkowska et al.
The Journal of biological chemistry, 292(16), 6633-6643 (2017-03-09)
Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a membrane-bound MMP that is highly expressed in cells with invading capacity, including fibroblasts and invasive cancer cells. However, pathways of MT1-MMP up-regulation are not clearly understood. A potential physiological stimulus for MT1-MMP expression
Molecular consequences of silencing mutant K-ras in pancreatic cancer cells: justification for K-ras-directed therapy.
Fleming, JB; Shen, GL; Holloway, SE; Davis, M; Brekken, RA
Molecular Cancer Research null
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