MABT1488

Anti-MBP (Citrulline R130) Antibody, clone 3C6

clone 3C6, from mouse

• 동의어(들): Myelin basic protein, Myelin A1 protein, Myelin membrane encephalitogenic protein, Citrullinated MBP
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 3C6, monoclonal
• 종 반응성: mouse, human
• 포장: antibody small pack of 25 μg
• 기술: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001020252
• UniProt 수납 번호: P02686
• 타겟 번역 후 변형: citrullination (Arg130)

설명

일반 설명

Myelin basic protein (UniProt: P02686; also known as MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein) is encoded by the MBP gene (Gene ID: 4155) in human. MBP is a homodimeric protein that is found in both the central and the peripheral nervous system. It is one of the most abundant protein component of the myelin membrane in the CNS and plays a role in both the formation and stabilization of this myelin membrane. It is responsible for adhesion of the cytosolic surfaces of multilayered compact myelin. MBP can interact with many polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and with negatively charged lipids. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. MBP expression begins abruptly in 14-16-week-old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. MBP citrullination by peptidylarginine deiminase (PAD) leads to incomplete protein-lipid bilayer interactions and increases its vulnerability to proteolytic enzymes, resulting in disorganization of the myelin sheath in the central nervous system. Citrullinated MBP (citMBP) has been suggested to be a hallmark of demyelination. MBP citrullination has frequently been demonstrated in various CNS disorders. citMBP at R25, R122, and R130 is reported in the brains of subjects with Creutzfeldt-Jakob disease (CJD). Clone 3C6 specifically recognizes citMBP at R130. (Ref.: Jang, B., et al. (2017). Mol. Neurobiol. 55(4); 3172-3184; Boggs, JM (2006). Cell. Mol. Life Sci. 63(17); 1945-1961).

특이성

Clone 3C6 is a mouse monoclonal antibody that detects human and murine myelin basic protein citrullinated on residue 130. It targets an epitope with in 19 amino acids from the C-terminal half.

면역원

KLH-conjugated linear peptide corresponding to 19 amino acids from isoform 5 of human Myelin Basic Protein with citrullination on residue 130.

애플리케이션

Anti-MBP (Citrulline R130), clone 3C6, Cat. No. MABT1488, is a mouse monoclonal antibody that detects Myelin basic protein citrullinated on residue 130 and has been tested for use in ELISA, Immunohistochemistry, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected MBP (Citrulline R130) in Immunohistochemistry applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MBP (Citrulline R130) in Myelin basic protein (MBP) treated with human PAD2 (Courtesy of Byungki Jang, Ph.D./Eun-Kyoung Choi, Ph.D., Ilsong Institute of Life Science, Hallym University, South Korea).

Western Blotting Analysis: A representative lot detected MBP (Citrulline R130) in Western Blotting applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

ELISA Analysis: A representative lot detected MBP (Citrulline R130) in ELISA applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Research Category
Cell Structure

품질

Evaluated by Western Blotting in GST-tagged myelin basic protein (MBP) treated with human PADI-2.

Western Blotting Analysis: 4 µg/mL of this antibody detected MBP (Citrulline R130) in GST-tagged myelin basic protein (MBP) treated with human Peptidyl Arginine Deiminase 2 (PAD-2)(200 ng for 4 h at 37°C).

표적 설명

~41 kDa observed; 18.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable