추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
AXO 49, monoclonal
종 반응성
Apis, porcine, snail, pig, sea urchin, protista, sheep, mouse, lemur, Drosophila, Paramecium, trout
반응하면 안 됨
Euglena, human (cilia)
기술
dot blot: suitable
immunofluorescence: suitable
western blot: suitable
동형
IgG1κ
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... TUBA1A(7846)
일반 설명
Several post-translational modifications of tubulin have been identified in eukaryotic cells. Glycylation is a poly-modification which occurs as lateral branching of glycine chains of variable length identified in axonemal tubulin of Paramecium cilia. This modification has been detected on tubulin and/or cilia/flagella of many unicellular and pluricellular organisms. The differential distribution of distinct polyglycylated tubulin isoforms between cytoplasmic and axonemal compartments indicates that polyglycylation levels of tubulin is highly regulated at the cell level. The AXO49 antibody is reactive mainly on axonemal tubulin of motile cilia and flagella which are polyglycylated. In some cases, reactivity may also be observed on other very stable microtubule networks. This antibody is useful for cilia and flagella detection.
특이성
This antibody recognizes the lateral connecting branches of a polymer, which contains at least 3 glycine residues. This antibody demonstrates cross reactivity against polyglycylated tubulin, as well as with other polyglycylated proteins .
면역원
Insoluble fraction of Paramecium cilia (Paramecium axonemes)
애플리케이션
Detect Tubulin using this mouse monoclonal antibody, Anti-pan polyglycylated Tubulin Antibody, clone AXO 49 validated for use in western blotting, Immunofluorescence & Dot Blot.
품질
Evaluated by Western Blotting on total cytoskeletal proteins of Paramecium. : A 1:50,000 dilution of this antibody detected polyglycylated Tubulin in 10 µg of Paramecium total cytoskeletal proteins.
표적 설명
~55 kDa observed
물리적 형태
Format: Purified
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Not finding the right product?
Try our 제품 선택기 도구.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
International journal of molecular sciences, 23(6) (2022-03-26)
A useful model for determining the mechanisms by which actin and actin binding proteins control cellular architecture is the Drosophila melanogaster process of spermatogenesis. During the final step of spermatogenesis, 64 syncytial spermatids individualized as stable actin cones move synchronously
Biology open, 10(8) (2021-08-07)
Primary cilia are compartmentalised from the rest of the cell by a ciliary gate comprising transition fibres and a transition zone. The ciliary gate allows the selective import and export of molecules such as transmembrane receptors and transport proteins. These
Frontiers in genetics, 13, 943197-943197 (2022-07-26)
Axonemal dynein motors are large multi-subunit complexes that drive ciliary movement. Cytoplasmic assembly of these motor complexes involves several co-chaperones, some of which are related to the R2TP co-chaperone complex. Mutations of these genes in humans cause the motile ciliopathy
Journal of molecular cell biology, 14(7) (2022-08-19)
Meiosis is essential for evolution and genetic diversity in almost all sexual eukaryotic organisms. The mechanisms of meiotic recombination, such as synapsis, have been extensively investigated. However, it is still unclear whether signals from the cytoplasm or even from outside
Haematologica, Online ahead of print (2020-12-18)
In specialised cells, the expression of specific tubulin isoforms and their subsequent post-translational modifications drive and coordinate unique morphologies and behaviours. The mechanisms by which β1-tubulin, the platelet and megakaryocyte (MK) lineage restricted tubulin isoform, drives platelet production and function
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.