MABS1727

Anti-CYB5R3 Antibody, clone 1D9.2

clone 1D9.2, from mouse

• 동의어(들): NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 1D9.2, monoclonal
• 종 반응성: human
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_000389
• UniProt 수납 번호: P00387
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CYB5R3(1727)

설명

일반 설명

NADH-cytochrome b5 reductase 3 (EC 1.6.2.2; UniProt P00387; also known as B5R, Cytochrome b5 reductase, Diaphorase-1) is encoded by the CYB5R3 (also known as DIA1) gene (Gene ID 1727) in human. Cytochrome b5 reductase is a flavoprotein that serves as electron donor for cytochrome b5, a ubiquitous electron carrier and participates in a variety of metabolic pathways, including desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. It is produced as two different isoforms that have different localizations. The membrane-bound form is found in somatic cells where it is anchored to the endoplasmic reticulum and mitochondrial membranes. The soluble form is found in erythrocytes. Mutations in the CYB5R3 gene result in deficiency in NADH-cytochrome b5 reductase+C20 in erythrocytes, which causes methemoglobinemia types I and II that are characterized by the presence of excessive amounts of methemoglobin in blood cells and their reduced capacity to carry oxygen. Ref.: Elahian, F., et al. (2014). Crit. Rev Biotechnol. 34, 134-143.

특이성

Target epitope is present in both the ER/mitochondria membrane-bound and cytosolic forms of human CYB5R3.

면역원

GST-tagged recombinant human CYB5R3 C-terminal fragment.

애플리케이션

Detect CYB5R3 using this mouse monoclonal Anti-CYB5R3 Antibody, clone 1D9.2, Cat. No. MABS1727, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected CYB5R3 in human kidney, skeletal muscle, and tonsil tissue sections.

품질

Evaluated by Western Blotting in HepG2 celll lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CYB5R3 in 10 µg of HepG2 cell lysate.

표적 설명

~34 kDa observed. 34.10 kDa (isoform 1 or M; Membrane-bound form with Met1 removed, a.a. 2-301), 31.26 kDa (Cytosolic form, a.a. 27-301), 31.63 kDa (isoform 2 or S), 38.23 kDa (isoform 3) calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1