추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
11/21-7-A8, monoclonal
종 반응성
human, mouse, rat
기술
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ATP5B(506)
일반 설명
ATP synthase subunit beta, mitochondrial (EC 3.6.3.14; UniProt P06576; also known as ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide, beta-F1-ATPase, Epididymis secretory protein Li 271, Mitochondrial ATP synthase beta subunit, Mitochondrial ATP synthetase beta subunit) is encoded by the ATP5B (also known as ATPMB, ATPSB, HEL-S-271) gene (Gene ID 506) in human. Mitochondrial ATP synthase produces ATP from ADP in the presence of a proton gradient generated by electron transport complexes. This ATPase contains two structural domains, F1-containing extramembrane catalytic core, and F0-containing the membrane proton channel that are linked via a central stalk and a peripheral stalk. Subunits alpha and beta form the catalytic code in F1. Tumor development requires the selection of cancer cells with a repressed biogenesis and functional activity of mitochondria. Both beta-F1-ATPase/GAPDH and beta-F1-ATPase/Hsp60 ratios are found to be significantly lower in tumors than the corresponding normal tissues. Studies conducted in human colon cancer cell line HCT116 show the involvement of AMPK (AMP-activated protein kinase) and GCN2 (general control non-derepressible 2; eIF2α kinase) in the onset of colon cancer progression by repressing of beta-F1-ATPase synthesis and promoting the abnormal bioenergetics of mitochondria.
면역원
His-tagged recombinant protein corresponding to human ATP Synthase subunit β.
애플리케이션
Immunocytochemistry Analysis: A representative lot immunostained mitochondrial tubular network in human breast cancer Hs578T cells (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
ELISA Analysis: A representative lot detected His-tagged full-length human ATP Synthase subunit β (beta-F1-ATPase) recombinant protein by direct ELISA (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Dot Blot Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) by Dot blot using His-tagged full-length human beta-F1-ATPase recombinant protein or HepG2 lysate (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) in human hepatoma HepG2, murine hepatoma Hepa 1-6, and normal rat liver epithelial C9 (Clone 9) cells.
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) expression in various cancer patients tissues (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) downregulation in HCT116 human colon cancer cells in response to AMPK pathway activation upon oligomycin or AICAR treatment (Martinez-Reyes, J., et al. (2012). Biochem J. 444(2):249-259).
ELISA Analysis: A representative lot detected His-tagged full-length human ATP Synthase subunit β (beta-F1-ATPase) recombinant protein by direct ELISA (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Dot Blot Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) by Dot blot using His-tagged full-length human beta-F1-ATPase recombinant protein or HepG2 lysate (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) in human hepatoma HepG2, murine hepatoma Hepa 1-6, and normal rat liver epithelial C9 (Clone 9) cells.
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) expression in various cancer patients tissues (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) downregulation in HCT116 human colon cancer cells in response to AMPK pathway activation upon oligomycin or AICAR treatment (Martinez-Reyes, J., et al. (2012). Biochem J. 444(2):249-259).
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-ATP Synthase subunit β Antibody, clone 11/21-7-A8 is validated for use in Western Blotting, Immunocytochemistry, ELISA and Dot Blot for the detection of ATP Synthase subunit β.
품질
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATP Synthase subunit β in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATP Synthase subunit β in 10 µg of HepG2 cell lysate.
표적 설명
~56 kDa observed
물리적 형태
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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