추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
10C5, monoclonal
분자량
calculated mol wt 79 kDa
observed mol wt ~57 kDa
정제법
using protein G
종 반응성
human, mouse
포장
antibody small pack of 100
기술
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
동형
IgG2bκ
에피토프 서열
N-terminal half
단백질 ID 수납 번호
UniProt 수납 번호
저장 온도
2-8°C
유전자 정보
human ... MAPT(4137)
특이성
Clone 10C5 is a mouse monoclonal antibody that detects Tau. It targets an epitope within 52 amino acids in the proline-rich region (PR-1) of human Tau.
면역원
His-tagged, recombinant, human Tau isoform 1N3R aggregates produced with arachidonic acid.
애플리케이션
Quality Control Testing
Evaluated by Western Blotting with His-tagged, Human recombinant Tau, isoform 1N4R. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, Human, recombinant Tau isoform 1N4R.
Tested Applications
Enzyme Immunoassay (ELISA): Serial dilutions from a representative lot detected Tau monomers or Tau aggregates induced with arachidonic acid in ELISA (Courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).
Western Blotting Analysis: A representative lot detected recombinant, human Tau isoform 2N4R monomers and aggregates, 2N3R monomers and aggregates, and mouse Tau monomers. (Courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).
Immunohistochemistry Applications: A representative lot detected Tau in Alzheimer′s diseased (AD) brain (superior temporal cortex, STG) tissue sections. Human disease sections had to be treated with phosphatase (dephosphorylates tau) to obtain reactivity suggesting tau in human AD is phosphorylated at its epitope. (Courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with His-tagged, Human recombinant Tau, isoform 1N4R. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, Human, recombinant Tau isoform 1N4R.
Tested Applications
Enzyme Immunoassay (ELISA): Serial dilutions from a representative lot detected Tau monomers or Tau aggregates induced with arachidonic acid in ELISA (Courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).
Western Blotting Analysis: A representative lot detected recombinant, human Tau isoform 2N4R monomers and aggregates, 2N3R monomers and aggregates, and mouse Tau monomers. (Courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).
Immunohistochemistry Applications: A representative lot detected Tau in Alzheimer′s diseased (AD) brain (superior temporal cortex, STG) tissue sections. Human disease sections had to be treated with phosphatase (dephosphorylates tau) to obtain reactivity suggesting tau in human AD is phosphorylated at its epitope. (Courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
표적 설명
Microtubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein Paired helical filament-tau (PHF-tau)) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Tau is a highly abundant protein found predominantly in the nervous system. Its primary role is to stabilize and support microtubule assembly, which are essential for maintaining the structure and function of nerve cells. It is present throughout the brain, particularly in regions involved in memory and cognition. MAPT gene consists of multiple exons that undergo alternative splicing, resulting in the generation of different isoforms that differ in the number and arrangement of repeats in the microtubule-binding domain. The expression of the MAPT transcripts varies and depends on the stage of neuronal maturation and neuron type. Aberrant aggregation and accumulation of tau protein have been linked to several neurodegenerative diseases collectively known as tauopathies. The most common tauopathy is Alzheimer′s disease, where hyperphosphorylated tau protein forms neurofibrillary tangles inside neurons. Other tauopathies include frontotemporal dementia, progressive supranuclear palsy, and cortico-basal degeneration. These diseases are characterized by cognitive decline, motor disturbances, and memory loss.
물리적 형태
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
재구성
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommended storage: +2°C to +8°C.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
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