MABN2302

Anti-ARHGAP11B Antibody, clone 3758-A37-5

• 동의어(들): Inactive Rho GTPase-activating protein 11B, Rho-type GTPase-activating protein 11B
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 3758-A37-5, monoclonal
• 분자량: calculated mol wt 30.25 kDa; observed mol wt ~30 kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100 μL
• 기술: immunofluorescence: suitable; western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: C-terminal
• 단백질 ID 수납 번호: NP_001034930
• UniProt 수납 번호: Q3KRB8
• 저장 온도: 2-8°C
• 유전자 정보: human ... ARHGAP11B(89839)

설명

특이성

Clone 3758-A37-5 is a mouse monoclonal antibody that detects Rho-type GTPase-activating protein 11B (ARHGAP11B). It targets an epitope within 21 amino acids from the C-terminal region.

면역원

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Rho-type GTPase-activating protein 11B (ARHGAP11B).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in lysate from COS-7 cells transfected with human ARHGAP11B.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ARHGAP11B in lysate from COS-7 cells transfected with human ARHGAP11B, but not in lysate from wild-type COS-7 cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected ARHGAP11B in Immunofluorescence application (Namba, T., et al. (2020). Neuron. 105(5):867-881.e9; Heide, M., et al. (2020). Science. 369(6503):546-550).

Western Blotting Analysis: A representative lot detected ARHGAP11B in Western Blotting applications (Namba, T., et al. (2020). Neuron. 105(5):867-881.e9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Inactive Rho GTPase-activating protein 11B (UniProt: Q3KRB8; also known as Rho-type GTPase-activating protein 11B) is encoded by the ARHGAP11B (also known as FAM7B1) gene (Gene ID: 89839) in human. ARHGAP11B is a mitochondrial matrix protein that is specifically expressed in radial glia cells of cortical neural stem and progenitor cells during neocortex development. It is preferentially expressed in apical and basal radial glia and is not detected in cortical neurons and cortical plate. It promotes amplification of basal progenitors in the subventricular zone, producing more neurons during fetal corticogenesis, thereby playing a key role in neocortex expansion. It promotes basal progenitor cell proliferation by inhibiting the mitochondrial permeability transition pore (mPTP). It delays the opening of the mPTP via interaction with ADP:ATP translocase, thereby increasing mitochondrial Ca2+ concentration and inducing glutamine catabolism, which is required for basal progenitor proliferation. ARHGAP11B does not exhibit GTPase activator activity and this lack of GTPase activator activity is essential for amplification of basal progenitors during neocortex development. (Ref.: Namba, T., et al. (2020). Neuron. 105(5); 867-881; Florio, M., et al. (2015). Science. 347(6229); 1465-1470).

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable