MABN1529

Anti-PRICKLE2 Antibody, clone 3B4.1

clone 3B4.1, from mouse

• 동의어(들): Prickle-like protein 2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 3B4.1, monoclonal
• 종 반응성: human, mouse
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_942559
• UniProt 수납 번호: Q7Z3G6
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PRICKLE2(166336)

설명

일반 설명

Prickle-like protein 2 (UniProt Q7Z3G6) is encoded by the PRICKLE2 (also known as EPM5) gene (Gene ID 166336) in human. Prickle2 is a nuclear membrane associated protein that belongs to a highly conserved Prickle, Espinas, Testin (PET) and Linl-1, Isl-1, and Mec-3 (LIM) domain-containing protein family. Prickle is part of the non-canonical Wnt signaling pathway that establishes planar cell polarity. It plays a critical role in neuron architecture and function. In mice it is shown to be highly expressed in the hippocampus region where it localizes to the post-synaptic density (PSD). It interacts the PSD-95 and NMDA receptor, the two proteins implicated in Autism spectrum disorders. Loss of Prickle2 is known to reduce number and size of miniature synaptic currents, indicating deficits in basal synaptic transmission. Mice deficient in Prickle2 exhibit lower seizure threshold and PRICKLE2 mutations in humans are associated with progressive myoclonic epilepsy type 5. Ref.: Sowers, LP., et al.. (2013), Mol. Psychiatry. 18, 1077-1089.

특이성

Clone 3B4.1 targets an internal PRICKLE2 epitope conserved between human and murine species.

면역원

GST-tagged recombinant human PRICKLE2 internal fragment.

애플리케이션

Anti-PRICKLE2 Antibody, clone 3B4.1, Cat. No. MABN1529, is a highly specific mouse monoclonal antibody that targets PRICKLE2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PRICKLE2 in mouse cerebral cortex, as well as in human pancreas, small intestine, and tonsil tissue sections.

Research Category
Neuroscience

품질

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PRICKLE2 in 10 µg of mouse brain tissue lysate.

표적 설명

~95 kDa observed. 95.62/95.30 kDa (human pro-/mature form) and 95.78/95.47 kDa (mouse pro-/mature form) calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable