생물학적 소스
rat
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
D3B2-2C1, monoclonal
종 반응성
human
포장
antibody small pack of 25 μg
기술
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG2bκ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... DNMT3B(1789)
일반 설명
DNA (cytosine-5)-methyltransferase 3B (Dnmt3B) is one of three mammalian enzymes that catalyze the addition of a methyl group to the 5’ carbon of cytosine residues in CpG dinucleotides. The Dnmt3b enzyme is involved in de novo methylation and the establishment of genome-wide DNA methylation patterns during development. DNA methylation has been linked to chromosome stability and gene expression in somatic cells; and to parental imprinting and X-chromosome inactivation in germ cells. Several studies have indicated that DNA methylation patterns are altered in various cancers.
면역원
Linear peptide corresponding to human Dnmt3b.
애플리케이션
Anti-Dnmt3b Antibody, clone D3B2-2C1 is a Rat Monoclonal Antibody for detection of Dnmt3b also known as DNA (cytosine-5)-methyltransferase 3B & has been validated in WB & IP.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate Dnmt3b in IP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
품질
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Dnmt3b in 10 µg of of HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Dnmt3b in 10 µg of of HeLa nuclear extract.
표적 설명
~110 kDa observed. Multiple isoforms between ~80-96 kDa are known to exist. A truncated protein at ~40 kDa may be observed in some cell lysates (Ostler, K. R., et al. (2007). 26(38): 5553-5563.). Uncharacterized bands may be observed at ~20 kDa and ~34 kDa in some cell lysates.
물리적 형태
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
분석 메모
Control
HeLa nuclear extract
HeLa nuclear extract
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Sadaf Harandi-Zadeh et al.
Antioxidants (Basel, Switzerland), 10(8) (2021-08-28)
Epigenetic aberrations are linked to sporadic breast cancer. Interestingly, certain dietary polyphenols with anti-cancer effects, such as pterostilbene (PTS), have been shown to regulate gene expression by altering epigenetic patterns. Our group has proposed the involvement of DNA methylation and
Katarzyna Lubecka et al.
Carcinogenesis, 37(7), 656-668 (2016-05-22)
DNA hypomethylation was previously implicated in cancer progression and metastasis. The purpose of this study was to examine whether stilbenoids, resveratrol and pterostilbene thought to exert anticancer effects, target genes with oncogenic function for de novo methylation and silencing, leading
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