MABE1526

Anti-ZCCHC11 (TUT4) Antibody, clone 9F6.1

clone 9F6.1, from mouse

• 동의어(들): Terminal uridylyltransferase 4, TUTase 4, Zinc finger CCHC domain-containing protein 11
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 9F6.1, monoclonal
• 종 반응성: human, mouse
• 기술: western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_056084
• UniProt 수납 번호: Q5TAX3
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ZCCHC11(23318)

설명

일반 설명

Terminal uridylyltransferase 4 (EC 2.7.7.52; UniProt Q5TAX3; also known as TUTase 4, Zinc finger CCHC domain-containing protein 11) is encoded by the ZCCHC11 (also known as KIAA0191, TUT4) gene (Gene ID 23318) in human. TUTase 4 is an uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. It is involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets and also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, and miR-143 and miR-200c. It is reported to catalyze the 3′ uridylation of miR-26A that targets IL6 transcript to abrogate the silencing of IL6 transcript and promote cytokine expression. It may also participate in toll-receptor signaling by suppressing TRAF6-dependent activation of NF-kappaB.

특이성

Clone 9F6.1 targets an epitope in the N-terminal region of ZCCHC11 (TUT4).

면역원

GST-tagged recombinant human ZCCHC11 (TUT4) N-terminal fragment.

애플리케이션

Anti-ZCCHC11 (TUT4), clone 9F6.1, Cat. No. MABE1526, is a highly specific mouse monoclonal antibody that targets ZCCHC11 (TUT4) and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 4 µg/mL from a representative lot detected ZCCHC11 (TUT4) in 10 µg of C2C12 mouse myoblast and Jurkat human T-cell lysates.

품질

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected ZCCHC11 (TUT4) in 10 µg of PC3 human prostate cancer cell lysate.

표적 설명

~185 kDa observed. 185.2/81.14 kDa (human isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified.

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable