MABC1814

Anti-CD44v6 Antibody, clone C44Mab-9

• 동의어(들): CDw44, Epican, Extracellular matrix receptor III (ECMR-III) GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, Phagocytic glycoprotein 1 (PGP-1), Phagocytic glycoprotein I (PGP-I)
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: C44Mab-9, monoclonal
• 분자량: calculated mol wt 82 kDa; observed mol wt ~N/A kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100
• 기술: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: N-terminal half
• 단백질 ID 수납 번호: NP_000601
• UniProt 수납 번호: P16070
• 저장 온도: 2-8°C
• 유전자 정보: human ... CD44(960)

설명

특이성

Clone C44Mab-9 is a mouse monoclonal antibody that detects CD44v6. It targets an epitope within 20 amino acids from the N-terminal half of the protein.

면역원

CHO cells transfected with PA16-tagged CD44v3 10

애플리케이션

Quality Control Testing

Evaluated by Flow Cytometry in a CHO cells overexpressing CD44v3-10.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD44v6 in one million CHO cells overexpressing CD44v3-10.

Tested Applications

Flow Cytometry Analysis: A representative lot detected CD44v4 in Flow Cytometry applications (Ejima, R., et al. (2023). Int. J. Mol. Sci. 24(4):4007).

Western Blotting Analysis: A representative lot detected CD44v4 in Western Blotting applications (Ejima, R., et al. (2023). Int. J. Mol. Sci. 24(4):4007).

Immunohistochemistry Applications: A representative lot detected CD44v6 in Immunohistochemistry applications (Ejima, R., et al. (2023). Int. J. Mol. Sci. 24(4):4007).

ELISA Analysis: A representative lot detected CD44v4 in ELISA applications (Ejima, R., et al. (2023). Int. J. Mol. Sci. 24(4):4007).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

CD44 antigen (UniProt: P16070; also known as CDw44, Epican, Extracellular matrix receptor III (ECMR-III), GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1 (PGP-1), Phagocytic glycoprotein I (PGP-I)) is encoded by the CD44 (also known as LHR, MDU2, MDU3, MIC4) gene (Gene ID: 960) in human. CD44 is a Type 1 transmembrane glycoprotein involved in cell adhesion, migration, and proliferation. It also plays a role in lymphocyte activation, tumor metastasis, and wound healing. It is encoded by the CD44 gene, and it is expressed in a wide range of cell types. The protein′s structure includes a hyaluronic acid (HA) binding domain, a stem region, and a variable extracellular domain. Alternative splicing may produce 19 isoforms. CD44 splice variants (CD44v) play a role in tumorigenesis and malignancy. It is also implicated in various other diseases, including autoimmune disorders, cardiovascular diseases and fibrosis. (Ref.: Suzuki, H., et al. (2023). Curr Issues Mol Biol. 45(3):1875-1888).

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable