추천 제품
생물학적 소스
mouse
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
SG2-D1a, monoclonal
종 반응성
mouse, sheep, rat
반응하면 안 됨
pig, rabbit, human, bovine, canine
제조업체/상표
Chemicon®
기술
immunohistochemistry: suitable
western blot: suitable
동형
IgG2b
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... DRD1(1812)
특이성
Dopamine Receptor D1A, no cross reactivity to D1B (D5) receptor. Immunoreactivity was found to be specific for mouse and rat D1A receptor. Human, pig, dog, rabbit, sheep and bovine samples were non-reactive by western analysis.
면역원
Recombinant rat D1a Receptor, carboxy terminus.
애플리케이션
Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a detects level of Dopamine D1A Receptor & has been published & validated for use in IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Western blot: 1:250-1:500 on unboiled membrane fractions from rat caudate tissue. Reactivity is also seen in perfrontal and temporal cortex. An identified band between 45-66kDa is observed (Luedtke, 1999).
Immunohistochemistry: 4% PFA fixed, vibratome sections, blocked with 3% NGS, 1% BSA with 0.2% triton X-100. Primary antibodies diluted onlyin PBS with excess blocking reagents.
Optimal working dilutions must be determined by the end user.
SUGGESTED WESTERN BLOT PROTOCOL
Solutions
10X Transfer buffer 1 liter
Tris 115.3 gm
Glycine 24.2 gm
[SDS 4 gm (may be omitted)]
For a 1X working solution: 1 liter
mix 700 mL water
200 mL MeOH
100 mL 10X TransferTBST
stock 1 liter
1M Tris pH 7.5 10 mL
5 M NaC 30 mL
10% Tween-20 5 mL
1. Run SDS-PAGE gel as desired.
2. Soak PVDF membrane (ImmobilonP from Millipore) in 100% methanol for 1-2 minutes to wet the membrane.
3. Soak membrane in transfer buffer (see below) until ready to set up blot transfer (>15 min).
4. Assemble gel-transfer sandwich with the blot toward the positive electrode.
5. Transfer protein from gel to membrane at 100 volts for 1-2 hours for small molecular weight proteins or 65 mA for 16 hours (overnight) for complete transfer of higher molecular weight proteins.
6. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).
7. Destain with deionized water.
8. Block with 5% non-fat milk (Marvel or Carnation) in TBS or PBS, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).
9. Incubation with first antibody overnight with gentle agitation at 4°C.
Optimal working dilutions and incubation time will need to be determined by the end user.
10. Wash at least 3 x 5 min. with TBST. From this stage, azide should be omitted.
11. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse antibody, for example Chemicon Catalog Number AP124P, diluted appropriately) 1 h at room temperature.
12. Wash at least 3 x 5 min. with TBST.
13. Perform ECL with commercial kit (femtoLUCENT, Chemicon Catalog Number 2078).
Immunohistochemistry: 4% PFA fixed, vibratome sections, blocked with 3% NGS, 1% BSA with 0.2% triton X-100. Primary antibodies diluted onlyin PBS with excess blocking reagents.
Optimal working dilutions must be determined by the end user.
SUGGESTED WESTERN BLOT PROTOCOL
Solutions
10X Transfer buffer 1 liter
Tris 115.3 gm
Glycine 24.2 gm
[SDS 4 gm (may be omitted)]
For a 1X working solution: 1 liter
mix 700 mL water
200 mL MeOH
100 mL 10X TransferTBST
stock 1 liter
1M Tris pH 7.5 10 mL
5 M NaC 30 mL
10% Tween-20 5 mL
1. Run SDS-PAGE gel as desired.
2. Soak PVDF membrane (ImmobilonP from Millipore) in 100% methanol for 1-2 minutes to wet the membrane.
3. Soak membrane in transfer buffer (see below) until ready to set up blot transfer (>15 min).
4. Assemble gel-transfer sandwich with the blot toward the positive electrode.
5. Transfer protein from gel to membrane at 100 volts for 1-2 hours for small molecular weight proteins or 65 mA for 16 hours (overnight) for complete transfer of higher molecular weight proteins.
6. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).
7. Destain with deionized water.
8. Block with 5% non-fat milk (Marvel or Carnation) in TBS or PBS, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).
9. Incubation with first antibody overnight with gentle agitation at 4°C.
Optimal working dilutions and incubation time will need to be determined by the end user.
10. Wash at least 3 x 5 min. with TBST. From this stage, azide should be omitted.
11. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse antibody, for example Chemicon Catalog Number AP124P, diluted appropriately) 1 h at room temperature.
12. Wash at least 3 x 5 min. with TBST.
13. Perform ECL with commercial kit (femtoLUCENT, Chemicon Catalog Number 2078).
물리적 형태
Format: Purified
Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
저장 및 안정성
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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