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생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
UIC2, monoclonal
종 반응성
primate, human
반응하면 안 됨
rat, mouse
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
동형
IgG2aκ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
유전자 정보
human ... ABCB1(5243)
관련 카테고리
일반 설명
UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE′s UIC2 Shift assay - see product ECM905).
특이성
P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)
면역원
Epitope: conformational extracellular epitope
Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine.
애플리케이션
Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2 detects level of MDR1 & has been published & validated for use in FC, IF, IP, IH, IH(P).
Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems.
Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 μg per 1 mL of cell lysate.
Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors.
Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 ug/ml for low Pgp expressors and 1 μg/mL for high Pgp expressors.
IHC on frozen and paraffin sections: 1 - 10 μg/mL.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 μg per 1 mL of cell lysate.
Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors.
Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 ug/ml for low Pgp expressors and 1 μg/mL for high Pgp expressors.
IHC on frozen and paraffin sections: 1 - 10 μg/mL.
Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Toxicology & Drug Resistance
Toxicology & Drug Resistance
물리적 형태
Format: Purified
The immunoglobulin was purified by protein A Sepharose™ chromatography and is presented as a liquid in in 0.1M PBS, containing 0.1% sodium azide as a preservative.
저장 및 안정성
Maintain at 2-8°C for up 12 months from date of receipt.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Marzena Szwed et al.
Cellular oncology (Dordrecht), 37(6), 421-428 (2014-11-21)
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Sarah M Valencia et al.
Journal of virology, 86(1), 2-10 (2011-10-28)
Epstein-Barr virus (EBV) uses different virus and cell proteins to enter its two major targets, B lymphocytes and epithelial cells. The routes that the virus takes into the two cell types are also different. To determine if these differences extend
Valentina Fonsato et al.
Stem cells (Dayton, Ohio), 30(9), 1985-1998 (2012-06-28)
Microvesicles (MVs) play a pivotal role in cell-to-cell communication. Recent studies demonstrated that MVs may transfer genetic information between cells. Here, we show that MVs derived from human adult liver stem cells (HLSC) may reprogram in vitro HepG2 hepatoma and
Activity of the Bcl-2 family inhibitor ABT-263 in a panel of small cell lung cancer xenograft models.
Shoemaker, AR; Mitten, MJ; Adickes, J; Ackler, S; Refici, M; Ferguson, D; Oleksijew et al.
Clinical cancer research : an official journal of the American Association for Cancer Research null
Levels of multidrug resistance (MDR1) P-glycoprotein expression by human breast cancer correlate with in vitro resistance to taxol and doxorubicin.
Mechetner, E, et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 4, 389-398 (1998)
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