MAB361

ANTI-TAU (MAPT) Antibody

CHEMICON^®, mouse monoclonal, Tau-5

• 동의어(들): G protein beta1/gamma2 subunit-interacting factor 1, Neurofibrillary tangle protein, Paired helical filament-tau, microtubule-associated protein tau, microtubule-associated protein tau, isoform 4
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 제품명: Anti-Tau Antibody, a.a. 210-241, clone Tau-5, ascites fluid, clone Tau-5, Chemicon^®
• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: Tau-5, monoclonal
• 종 반응성: mouse, human, rat, bovine
• 제조업체/상표: Chemicon^®
• 기술: immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NM_005910.3
• UniProt 수납 번호: P10636
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MAPT(4137) ; mouse ... Mapt(17762) , Mapt(281296) ; rat ... Mapt(29477)

설명

일반 설명

Microtubule Associated Proteins, or MAPS, bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosphorylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, I, II. Type II MAP includes MAP2, MAP4, and tau and are found in nervous tissue. Six tau isoforms exist in brain tissue, and they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (allowing it to bind to the negatively-charged microtubule). The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.

특이성

Tau from bovine, rat and human.

면역원

Bovine tau

Epitope: a.a. 210-241

애플리케이션

Anti-Tau Antibody, a.a. 210-241, clone Tau-5 detects level of Tau & has been published & validated for use in IH & WB.

Immunohistochemistry:
A previous lot of this antibody was used in immunohistochemistry.

Immunoblot:
Recognizes phosphorylated and non-phosphorylated isoforms of tau ranging in size from 45-68 kDa.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

품질

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Tau on 10 μg of PC12 lysates.

표적 설명

45-68 kDa

결합

Replaces: MAB10417

물리적 형태

Unpurified

Unpurified mouse monoclonal IgG1 ascites fluid containing no preservatives.

저장 및 안정성

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
Brain Tissue, PC12 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable