Met5- and Leu5-enkephalin. Displays about 40% cross-reactivity with C-terminal extended Met-enkephalin hexapeptides and 7% cross-reactivity with the extended heptapeptide (-Arg-Phe-OH) but does not recognize pro-enkephalin or other endogenous peptides (Cuello, 1984). By immunohistochemistry the antibody lables all sites that are known to exppress enkephalin and shows no cross-reactivity to beta-endorphin or dynorphin (Cuello, 1984; MacMillan and Cuello, 1986).
면역원
Leu5-enkephalin conjugated to BSA.
애플리케이션
Detect Enkephalin using this Anti-Enkephalin Antibody, clone NOC1 validated for use in IC, IH.
Immunohistochemistry: 1:100-1:400 on 10 μm cryostat sections of rat and guinea-pig brain stem using fluorescently labled secondary antibodies for detection (Cuello, 1984; MacMillan and Cuello, 1986).
Immunocytochemistry: 1:300 on primary cultures of rat striatal and hippocampal neurons using a Cy2 or Cy3 conjugated secondary antibody for detection (Saudou, 1998).
Radioimmunoassay (Cuello, 1984)
Optimal working dilutions must be determined by the end user.
Research Category Neuroscience
Research Sub Category Neuroinflammation & Pain
Hormones & Receptors
물리적 형태
Lyophilized Supernatant, preservative free.
저장 및 안정성
Lyophilized antibody can be kept at 2-8°C for up to 3 months and should be kept at -20°C for long term storage. Reconstitute with 100μL sterile DI water. Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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