MAB3222

Anti-BrdU Antibody, clone IIB5

clone IIB5, Chemicon^®, from mouse

• 동의어(들): BrdU
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: IIB5, monoclonal
• 종 반응성(상동성에 의해 예측): all
• 제조업체/상표: Chemicon^®
• 기술: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin)
• 동형: IgG1
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified

설명

일반 설명

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

특이성

Reacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.

면역원

A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.

애플리케이션

Anti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P).

Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. See:

http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.

Flow Cytometry: 1:100-1:200; acid methanol treatment required.

Optimal working dilutions must be determined by the end user.

In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

물리적 형태

Format: Purified

Protein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide.

Protein A purified

저장 및 안정성

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

분석 메모

Control
After incorporation of BrdU, all DNA containing species

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable