제품 MAB3140은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.
추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
4E9, monoclonal
종 반응성
amphibian, fish, avian, vertebrates (including including fish, amphibians, birds and mammals)
종 반응성(상동성에 의해 예측)
mouse, mammals, rat
제조업체/상표
Chemicon®
기술
western blot: suitable
동형
IgG1
적합성
not suitable for immunoprecipitation
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... SLC9A1(6548)
일반 설명
Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue-specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.
특이성
Na+/H+ exchanger, isoform NHE1
면역원
Epitope: C-terminus
MBP fusion protein containing the entire C-terminal, hydrophylic domain of porcine NHE1.
애플리케이션
Anti-Na+/H+ Exchanger-1 Antibody, C-terminus, clone 4E9 detects level of Na+/H+ Exchanger-1 & has been published & validated for use in WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Works poorly for immunohistochemistry and immunocytochemistry.
Not recommended for immunoprecipitation.
Immunoblotting:
1:1,000 dilution used on a previous lot.
Optimal working dilutions must be determined by the user.
Not recommended for immunoprecipitation.
Immunoblotting:
1:1,000 dilution used on a previous lot.
Optimal working dilutions must be determined by the user.
품질
Routinely evaluated by Western Blot on Human Kidney lysates.
Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.
Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.
표적 설명
~100-110 kDa
물리적 형태
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 liquid in buffer containing 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide
저장 및 안정성
Stable for 1 year at 2-8ºC from date of receipt.
분석 메모
Control
Kidney tissue (basolateral membrane)
Kidney tissue (basolateral membrane)
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
C Juel et al.
The Journal of physiology, 548(Pt 2), 639-648 (2003-03-04)
Chronic hypoxia is accompanied by changes in blood and skeletal muscle acid-base control. We hypothesized that the underlying mechanisms include altered protein expression of transport systems and the enzymes involved in lactate, HCO3- and H+ fluxes in skeletal muscle and
Lionel Blanc et al.
American journal of hematology, 90(3), 235-241 (2014-12-18)
Genetic ablation of the ferrireductase STEAP3, also known as TSAP6, leads to severe microcytic and hypochromic red cells with moderate anemia in the mouse. However, the mechanism leading to anemia is poorly understood. Previous results indicate that TSAP6/Steap3 is a
Sebastiano Vilella et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 13(4), 207-214 (2003-07-24)
In thyroid cells, the intracellular pH plays a key role in the control of the iodide uptake, since iodide accumulation is associated with an intracellular acidification. In the present paper we studied the kinetic proprieties of the Na(+)/H(+) antiporter (NHE)
Casper Skovgaard et al.
Journal of applied physiology (Bethesda, Md. : 1985), 124(2), 259-267 (2017-09-25)
The effect of tapering following a period of high-volume sprint interval training (SIT) and a basic volume of aerobic training on performance and muscle adaptations in moderately trained runners was examined. Eleven (8 men, 3 women) runners [maximum oxygen uptake
Cian McGinley et al.
Experimental physiology, 101(12), 1565-1580 (2016-10-01)
What is the central question of this study? Following a training intervention, how is the interpretation of adaptations in skeletal muscle H+ transporters influenced by biopsy timing in the context of individual protein and mRNA kinetics after the final exercise
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
