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Merck
모든 사진(2)

주요 문서

MAB1904

Sigma-Aldrich

Anti-Laminin-1 A&B chains Antibody

CHEMICON®, rat monoclonal, AL-2

동의어(들):

laminin alpha1, laminin beta1

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
제품 MAB1904은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.

제품명

Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2, clone AL-2, Chemicon®, from rat

생물학적 소스

rat

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

AL-2, monoclonal

종 반응성

mouse

제조업체/상표

Chemicon®

기술

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG1κ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

mouse ... Lama1(16772)

일반 설명

Laminins are secreted glycoproteins incorporated into cell-associated extracellular matrices and an integral part of the structural scaffolding in most animal tissues. The laminin protein is made up of three polypeptide chains; α, β and γ, accounting for its flexibility in connecting various molecules. When originally characterized, laminin-1 clone AL-2 reacted with Engelbreth-Holm-Swarm (EHS) mouse laminin A & B chains on the long arm near the cross region, (Skubitz, 1987). More recently, investigations, (Schèele, 2006) have shown that the clone binds laminin β1 under reducing conditions as well as native ELISAs and that the affinity is increased by the presence of the laminin α1 chain. Thus there is significant laminin β1 reactivity with clone AL-2.

특이성

Other species have not been tested.
Reacts with EHS mouse laminin A & B chains on the long arm near the cross region.

면역원

Epitope: Near cross region
Laminin isolated from EHS mouse sarcoma.

애플리케이션

Detect Laminin-1 A&B chains using this Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2 validated for use in ELISA, IF, IH(P), IP & WB.
Immunofluorescence:
An independent laboratory (Schèele, S., 2006) has verified anti-laminin clone AL-2 to work using immunofluorescence techniques on mouse muscle tissue, including both transgenic and wild-type tissues.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

품질

Evaluated on a representative lot by Western blot on purified mouse laminin-1.

표적 설명

~200 kDa

물리적 형태

Format: Purified
Protein G Purified
Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.

저장 및 안정성

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

George S Karagiannis et al.
Molecular oncology, 8(2), 178-195 (2013-11-26)
It has been suggested that cancer-associated fibroblasts (CAFs) positioned at the desmoplastic areas of various types of cancer are capable of executing a migratory program, characterized by accelerated motility and collective configuration. Since CAFs are reprogrammed derivatives of normal progenitors
H Kurata et al.
The Journal of allergy and clinical immunology, 103(5 Pt 2), S471-S484 (1999-05-18)
Adenoviruses have several specific features useful for gene therapy. They infect various lineages of cells irrespective of cell cycle status. However, the exact mechanism of their infection and in vivo kinetics as a gene expression vector have not been elucidated.
Ghaidaa Kashgari et al.
Developmental cell, 52(6), 764-778 (2020-02-29)
The fusion of digits or toes, syndactyly, can be part of complex syndromes, including van der Woude syndrome. A subset of van der Woude cases is caused by dominant-negative mutations in the epithelial transcription factor Grainyhead like-3 (GRHL3), and Grhl3-/-mice
Jangwook P Jung et al.
Biomacromolecules, 14(9), 3102-3111 (2013-07-24)
Limiting the precise study of the biochemical impact of whole molecule extracellular matrix (ECM) proteins on stem cell differentiation is the lack of 3D in vitro models that can accommodate many different types of ECM. Here we sought to generate
Raven Diacou et al.
Cell reports, 25(9), 2510-2523 (2018-11-30)
Gene regulation of multipotent neuroretinal progenitors is partially understood. Through characterizing Six3 and Six6 double knockout retinas (DKOs), we demonstrate Six3 and Six6 are jointly required for the maintenance of multipotent neuroretinal progenitors. Phenotypes in DKOs were not found in

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