추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
클론
D5/16B4, monoclonal
종 반응성
rat, mouse, human
제조업체/상표
Chemicon®
기술
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... KRT5(3852)
일반 설명
The cytokeratins are a family of intermediate filaments found in almost all epithelial cells. They are divided into four subclasses depending on their patterns of reactivity and electrophoretic characteristics and are also arranged I a numbered series of 20 or more acoring to molecular weight and isoelectric point (Quinlan et al. 1985). Types 5 and 6 of cytokeratin (CK5/6) are in high frequency in mesothelial cells, as opposed to cells of adenocarcinoma. CK5/6 has been shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung and Bowen′s disease. It does not react with pulmonary adenocarcinoma.
특이성
Expected to cross-react with mouse and rat.
Reacts with human cytokeratin 5 on immunoblots of cytoskeletal preparations of epidermis and non-cytokeratinizing epithelium (Abstract: European Symposium of the Biology of the Cytoskeleton, Helsinki, 18-21, 6, 89, 1989) and on tissue sections. Shows reactivity with cytokeratin 6 and weak reactivity with cytokeratin 4 on immunoblots. No cross-reactivity with cytokeratins 1, 7, 8, 10, 13, 14, 18 or 19. MAB1620 recognizes basal cells and a part of the stratum spinosum in the normal pavement epithelium. The antibody may be useful for distinction of low differentiated pavement epithelium carcinoma and adenocarcinoma. The antibody works on formalin fixed tissues.
면역원
Purified cytokeratin 5 (Exp. Cell Res. (1986) 162:114; J. Invest. Dermatology (1987) 88:191).
애플리케이션
Anti-Cytokeratin 5 Antibody, 6, clone D5/16B4 is an antibody against Cytokeratin 5 for use in IF, IH(P) & WB.
Immunohistochemistry (frozen or paraffin sections):
A 1:50-1:100 dilution from a previous lot was used for paraffin, high-temperature antigen retrieval required (Pressure cooker, Citrate/EDTA buffer pH 6.0, 4 minutes at full pressure).
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Western blot:
Recognizes a 58kDa and 56kDa band corresponding to cytokeratin 5 and 6.
Optimal working dilutions must be determined by end user.
A 1:50-1:100 dilution from a previous lot was used for paraffin, high-temperature antigen retrieval required (Pressure cooker, Citrate/EDTA buffer pH 6.0, 4 minutes at full pressure).
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Western blot:
Recognizes a 58kDa and 56kDa band corresponding to cytokeratin 5 and 6.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
품질
Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected cytokeratin 5/6 on 10 ug of A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected cytokeratin 5/6 on 10 ug of A431 lysates.
표적 설명
Cytokeratin 4 (59 kDa), Cytokeratin 5 (58 kDa) and Cytokeratin 6 (56 kDa).
물리적 형태
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
저장 및 안정성
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
분석 메모
Control
A431 cell lysate, human bladder tumor tissue.
A431 cell lysate, human bladder tumor tissue.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Variation and frequency of cytokeratin polypeptide patterns in human squamous non-keratinizing epithelium.
Wild, G A and Mischke, D
Experimental Cell Research, 162, 114-126 (1986)
D Allende et al.
Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus, 26(3), 314-318 (2012-09-27)
Patients with Barrett's esophagus are at risk for dysplasia and esophageal adenocarcinoma. Although surgery was the mainstay treatment for Barrett's dysplasia and cancer, patients with high-grade dysplasia and early cancers now have several nonsurgical treatment options. Most of the endoscopic
Julien Parra et al.
Scientific reports, 7, 43682-43682 (2017-03-09)
Post-translational modifications (PTMs) are essential processes conditioning the biophysical properties and biological activities of the vast majority of mature proteins. However, occurrence of several distinct PTMs on a same protein dramatically increases its molecular diversity. The comprehensive understanding of the
Felipe C Geyer et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 23(7), 951-960 (2010-05-11)
Breast adenosquamous carcinomas are rare tumours characterized by well-developed gland formation intimately admixed with solid nests of squamous cells immersed in a highly cellular spindle cell stroma. A low-grade variant has been described that is associated with a better prognosis.
Immunohistochemical Predictors of Bone Metastases in Breast Cancer Patients.
Winczura, P; Sosinska-Mielcarek, K; Duchnowska, R; Badzio, A; Lakomy, J; Majewska et al.
Pathology Oncology Research null
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