Extending the primary incubation step beyond the specified time in the kit protocol is not something that has been tested. A longer incubation time could potentially lead to decreased performance, including poor recovery or increased variability in assay characteristics. It may also result in higher nonspecific binding, leading to elevated signals in background wells and a loss of sensitivity.
HTH17MAG-14K
MILLIPLEX® Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay
Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
동의어(들):
Human Th17 cytokine multiplex kit, Luminex® human Th17 cytokine immunoassay, Millipore human Th17 cytokine panel
로그인조직 및 계약 가격 보기
모든 사진(2)
About This Item
UNSPSC 코드:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
추천 제품
Quality Level
종 반응성
human
제조업체/상표
Milliplex®
assay range
accuracy: 79-107%
standard curve range: 1-5000 pg/mL
(IL-10)
standard curve range: 10-40,000 pg/mL
(IFNγ)
standard curve range: 12-50,000 pg/mL
(IL-17A & IL-2)
standard curve range: 24-100,000 pg/mL
(IL-17 & IL-4)
standard curve range: 3-10,000 pg/mL
(IL-6 & TNFα)
standard curve range: 336-1,500,000 pg/mL
(IL-23)
standard curve range: 37-150,000 pg/mL
(IL-22 & TNFβ)
standard curve range: 488-2,000,000 pg/mL
(IL-17E/IL-25)
standard curve range: 49-200,000 pg/mL
(IL-28a & IL-31)
standard curve range: 5-20,000 pg/mL
(MIP-3α, IL-1β, IL-12 (p70), IL-15, IL-21 & IL-33)
standard curve range: 6-25,000 pg/mL
(IL-5)
standard curve range: 61-250,000 pg/mL
(GM-CSF & IL-27)
standard curve range: 7-30,000 pg/mL
(IL-13)
standard curve range: 9-35,000 pg/mL
(IL-9)
기술
multiplexing: suitable
검출 방법
fluorometric (Luminex xMAP)
배송 상태
wet ice
일반 설명
CD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFNγ, secrete IFNγ that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation.
Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn′s disease, and autoimmune uveitis. In addition, Th17 cells play an important role in tumor development, progression, and metastasis, potentially in both promoting and inhibiting tumor growth.
MILLIPLEX® Human TH17 Bead Panel is a 25-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α and TNFβ.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn′s disease, and autoimmune uveitis. In addition, Th17 cells play an important role in tumor development, progression, and metastasis, potentially in both promoting and inhibiting tumor growth.
MILLIPLEX® Human TH17 Bead Panel is a 25-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α and TNFβ.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
특이성
Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
애플리케이션
- Analytes: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβ
- Recommended Sample type: Serum, plasma, and cell culture samples
- Recommended Sample dilution: Neat
- Assay Run Time: Overnight
- Research Category: Inflammation & Immunology
특징 및 장점
Design your multiplex kit by choosing available analytes within this panel.
저장 및 안정성
Recommended storage for kit components is 2 - 8°C.
기타 정보
Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.
법적 정보
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
표적 기관
Respiratory Tract
Storage Class Code
10 - Combustible liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
E Suzanne Cohen et al.
Nature communications, 6, 8327-8327 (2015-09-15)
In response to infections and irritants, the respiratory epithelium releases the alarmin interleukin (IL)-33 to elicit a rapid immune response. However, little is known about the regulation of IL-33 following its release. Here we report that the biological activity of
Marta Czesnikiewicz-Guzik et al.
European heart journal, 40(42), 3459-3470 (2019-09-11)
Inflammation is an important driver of hypertension. Periodontitis is a chronic inflammatory disease, which could provide a mechanism for pro-hypertensive immune activation, but evidence of a causal relationship in humans is scarce. We aimed to investigate the nature of the
Danielle W Lowe et al.
Pediatric research, 82(1), 55-62 (2017-01-19)
Vitamin D has neuroprotective and immunomodulatory properties, and deficiency is associated with worse stroke outcomes. Little is known about effects of hypoxia-ischemia or hypothermia treatment on vitamin D status in neonates with hypoxic-ischemic encephalopathy (HIE). We hypothesized vitamin D metabolism
Olivier Fogel et al.
The Journal of allergy and clinical immunology, 142(1), 258-268 (2017-09-17)
An interferon signature is involved in the pathogenesis of primary Sjögren syndrome (pSS), but whether the signature is type 1 or type 2 remains controversial. Mouse models and genetic studies suggest the involvement of TH1 and type 2 interferon pathways.
Gunita Deksne et al.
Vector borne and zoonotic diseases (Larchmont, N.Y.), 21(1), 1-5 (2020-09-29)
The zoonotic parasite Dirofilaria repens has spread toward north in Europe, and cases of autochthonous dirofilariosis caused by D. repens have emerged in the Baltic countries Estonia, Latvia, and Lithuania. We conducted a review on the emergence of dirofilariosis in
관련 콘텐츠
See how multiplexing the inflammation signaling pathway with MILLIPLEX® inflammation assays or cell signaling assays can help researchers bridge the gap between immunology and cell signaling, including investigating T cell signaling, Th Cell differentiation, inflammatory response signaling, and sepsis signaling.
MILLIPLEX® 분석과 같은 면역학 멀티플렉스 분석을 통해 연구자들은 더 많은 면역 바이오마커를 동시에 조사할 수 있어 시간과 자원을 절약합니다. 이는 수많은 분석 물질이 다양한 경로에서 역할을 하는 면역학 연구에 특히 중요합니다.
Immunology multiplex assays, like MILLIPLEX® assays, allow researchers to investigate more immune biomarkers simultaneously, saving time and resources.
Discover the benefits of MILLIPLEX® multiplex assays, based on Luminex® xMAP® multiplex assay technology, that provide consistent, high-quality results and see how these multiplex biomarker Luminex® assays are being used to advance research.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
