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Merck
모든 사진(1)

주요 문서

CP18

Sigma-Aldrich

Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11)

liquid, clone II-4C11, Calbiochem®

동의어(들):

Collagen Type II antibody

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.43
클론:
II-4C11, monoclonal
application:
종 반응성:
rat, human, bovine, rabbit
citations:
42

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

II-4C11, monoclonal

양식

liquid

포함

≤0.1% sodium azide as preservative

종 반응성

rat, human, bovine, rabbit

제조업체/상표

Calbiochem®

저장 조건

OK to freeze
avoid repeated freeze/thaw cycles

동형

IgG1

배송 상태

wet ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... COL2A1(1280)

일반 설명

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-X-63-Ag8-LUI mouse myeloma cells. Recognizes the ~130 kDa type II collagen protein.
Recognizes the ~130 kDa type II collagen protein. May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected.
This Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) is validated for use in Frozen Sections for the detection of Collagen Type II (Ab-1).

면역원

Human
purified collagen type II

애플리케이션

Frozen Sections (1-10 µg/ml)

Immunoblotting (1-5 µg/ml, see application references)

Paraffin Sections (1-10 µg/ml, see application references)

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

물리적 형태

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

재구성

Following initial thaw, aliquot and freeze (-20°C).

기타 정보

Kumagai, J. et al. 1994 J. Anat.185, 278.
Kivrikko, K,I. 1993 Ann. Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell. Biol.114, 1307.
Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected. This antibody may also react with other classes of collagen at a concentration higher than 1 µg/ml. In immunoblotting, a major band of ~130 kDa is obtained. Antibody should be titrated for optimal results in individual systems.

법적 정보

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Chang Qing et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 44(4), 303-310 (2011-04-14)
Chondrocytes and bone marrow mesenchymal stem cells (BMSCs) are frequently used as seed cells in cartilage tissue engineering. In the present study, we determined if the co-culture of rabbit articular chondrocytes and BMSCs in vitro promotes the expression of cartilaginous
Zheng-Zheng Zhang et al.
Science translational medicine, 11(487) (2019-04-12)
Reconstruction of the anisotropic structure and proper function of the knee meniscus remains an important challenge to overcome, because the complexity of the zonal tissue organization in the meniscus has important roles in load bearing and shock absorption. Current tissue
María Satué et al.
Scientific reports, 9(1), 10153-10153 (2019-07-14)
Intra-articular (IA) injection of mesenchymal stem cells (MSCs) promotes articular cartilage repair. However, cell fate and action after transplantation remain unclear. This study aimed at evaluating the biodistribution and efficacy of MSCs after IA injection. We used an immunocompetent, dual
En-Rung Chiang et al.
PloS one, 11(2), e0149835-e0149835 (2016-02-26)
Mesenchymal stem cell (MSC)-based therapies may aid in the repair of articular cartilage defects. The purpose of this study was to investigate the effects of intraarticular injection of allogeneic MSCs in an in vivo anterior cruciate ligament transection (ACLT) model
En-Rung Chiang et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(6), 1440-1450 (2019-05-08)
Intervertebral discs (IVDs) are important biomechanical components of the spine. Once degenerated, mesenchymal stem cell (MSC)-based therapies may aid in the repair of these discs. Although hypoxic preconditioning enhances the chondrogenic potential of MSCs, it is unknown whether bone marrow

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