추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
6B3, monoclonal
종 반응성
human, chicken, mouse, salamander
제조업체/상표
Chemicon®
기술
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... COL2A1(1280)
특이성
In Western blotting (but not ELISA), MAB8887 recognizes both a1(II) and a3(XI) chains which have identical primary structure. In Western blotting, MAB8887 reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin-digested type II collagen. It will not react with intact Collagen II under traditional conditions.
Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Immunoblotting of CNBr peptides of collagen II shows that MAB8887 reacts with CB11 (25kDa) fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule.
In pepsin solublized collagen II, MAB8887 reacts with a 95-97 kDa fragment, as well as, the native single chain of 120kDa. If propeptides are present, MAB8887 will detect a 200kDa fragment.
Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Immunoblotting of CNBr peptides of collagen II shows that MAB8887 reacts with CB11 (25kDa) fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule.
In pepsin solublized collagen II, MAB8887 reacts with a 95-97 kDa fragment, as well as, the native single chain of 120kDa. If propeptides are present, MAB8887 will detect a 200kDa fragment.
면역원
Purified type II collagen; obtained from adult chicken sternum by limited pepsinization.
애플리케이션
Anti-Collagen Type II Antibody, clone 6B3 is an antibody against Collagen Type II for use in IF, IH & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Western Blot (Mayne, 1994)
Immunohistochemistry (Formalin/Paraffin): 1-2 μg/mL, 30 minutes at room temperature. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1mg/mL in Tris HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37ºC.
Immunofluorescence
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry (Formalin/Paraffin): 1-2 μg/mL, 30 minutes at room temperature. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1mg/mL in Tris HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37ºC.
Immunofluorescence
ELISA
Optimal working dilutions must be determined by end user.
표적 설명
120 kDa
물리적 형태
Format: Purified
Protein G Purified
Purified immunoglobulin in 10 mM PBS, pH 7.4 with 0.2% BSA and 0.09% sodium azide.
저장 및 안정성
Maintain at 2–8°C in undiluted aliquots for up to 12 months
분석 메모
Control
Cartilage in lung or fetus
Cartilage in lung or fetus
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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