추천 제품
생물학적 소스
human
분석
>95% (total protein)
양식
liquid
특이 활성도
>1200 mU/mg (specific activity of activated MMP-9 after trypsin activation)
제조업체/상표
Chemicon®
농도
0.2 mg/mL
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
유전자 정보
human ... MMP9(4318)
일반 설명
When measured with the peptide substrate dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg [Masui et al., 1977] the specific activity of MMP-9 monomer
애플리케이션
ACTIVATION:
An aliquot of 10 μL MMP-9 monomer is mixed with 20 μL trypsin solution (see below) and activation buffer in a total volume of 100 μL. The mixture is incubated for 20 min at 37°C. Thereafter trypsin is inhibited by addition of 10 μL aprotinin solution.
Trypsin solution: 0.50 mg TPCK-trypsin/mL activation buffer. The solution is stored in aliquots at -20°C.
Activation Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2.
Aprotinin solution: 1 mg aprotinin/mL activation buffer. The solution is stored at -20°C.
An aliquot of 10 μL MMP-9 monomer is mixed with 20 μL trypsin solution (see below) and activation buffer in a total volume of 100 μL. The mixture is incubated for 20 min at 37°C. Thereafter trypsin is inhibited by addition of 10 μL aprotinin solution.
Trypsin solution: 0.50 mg TPCK-trypsin/mL activation buffer. The solution is stored in aliquots at -20°C.
Activation Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2.
Aprotinin solution: 1 mg aprotinin/mL activation buffer. The solution is stored at -20°C.
단위 정의
Specific Activity: where 1 U is the activity that hydrolyzes 1 mmol peptide (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Dpa-Ala-Arg) within 1 minute under the assay conditions described by Knight ,et al
물리적 형태
Provided as a liquid in 50 mM Tris-HCl, pH 7.0, 200 mM NaCl, 5 mM CaCl2, ,1 μM ZnCl2, 0.05% Brij-35, 0.05% NaN3.
저장 및 안정성
Maintain frozen at -70°C in undiluted aliquots. The enzyme may be stored at -20°C for several without significant loss of activity. Repeated freezing and thawing should be avoided.
분석 메모
MMP-9 monomer appears as a major band at 92 kDa in non-reducing SDS-PAGE
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
M(r) 92,000 type IV collagenase is increased in plasma of patients with colon cancer and breast cancer.
Zucker, S, et al.
Cancer Research, 53, 140-146 (1993)
Synthetic substrates for vertebrate collagenase.
Masui, Y, et al.
Biochemical Medicine, 17, 215-221 (1977)
Secretion of collagenolytic enzymes by human polymorphonuclear leukocytes.
Hibbs, M S, et al.
Collagen and Related Research, 4, 467-477 (1984)
I E Collier et al.
Genomics, 9(3), 429-434 (1991-03-01)
The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. Two members of this family, collagenase and stromelysin, have previously been localized to the long arm of chromosome 11. Here we assign both of
Matrix metalloproteinases (MMP-2 and MMP-9) of the oral cavity: cellular origin and relationship to periodontal status.
Makela, M, et al.
Journal of Dental Research, 73, 1397-1406 (1994)
활성 필터
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