추천 제품
생물학적 소스
donkey
Quality Level
결합
biotin conjugate
항체 형태
affinity purified immunoglobulin
항체 생산 유형
secondary antibodies
클론
polyclonal
종 반응성
goat
제조업체/상표
Chemicon®
농도
1 mg/mL
기술
ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
배송 상태
wet ice
타겟 번역 후 변형
unmodified
일반 설명
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
특이성
Specific for Goat IgG.
애플리케이션
Donkey anti-Goat IgG Antibody, biotin-SP conjugate, Species Adsorbed detects level of Donkey Goat IgG & has been published & validated for use in ELISA, FC, IF, IH & WB.
EIA: 1:20,000-1:400,000
Western blots using enzyme-conjugated streptavidin: 1:20,000-1:400,000.
Immunohistochemistry: 1:500-1:5,000
Flow cytometry: 1:200-1:1,000
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000
Optimal working dilutions must be determined by end user.
Western blots using enzyme-conjugated streptavidin: 1:20,000-1:400,000.
Immunohistochemistry: 1:500-1:5,000
Flow cytometry: 1:200-1:1,000
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000
Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Secondary Antibodies Adsorbed for Dual Labeling
물리적 형태
Immunoaffinity Purified and Immunodepletion
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.
RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.
저장 및 안정성
Maintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Bastian Henkel et al.
Chemical senses, 42(1), 25-35 (2016-09-23)
In most mammals, the vomeronasal system detects a variety of (semio)chemicals that mediate olfactory-driven social and sexual behaviors. Vomeronasal chemosensation depends on G protein-coupled receptors (V1R, V2R, and FPR-rs) that operate at remarkably low stimulus concentrations, thus, indicating a highly
Yulia Shamis et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(16), E2020-E2029 (2015-04-08)
Zfp57 is a maternal-zygotic effect gene that maintains genomic imprinting. Here we report that Zfp57 mutants exhibited a variety of cardiac defects including atrial septal defect (ASD), ventricular septal defect (VSD), thin myocardium, and reduced trabeculation. Zfp57 maternal-zygotic mutant embryos
Y Y Yu et al.
The Journal of international medical research, 38(2), 583-592 (2010-06-03)
The effect of 2,2'-dipyridyl (DP) on cerebral vasospasm was investigated in a double-injection rabbit model of subarachnoid haemorrhage (SAH). Thirty-six animals were divided between four groups: control (sham-operated), SAH (model alone), SAH + DP (the SAH model in which DP
Shang-Cheng Huang et al.
Scientific reports, 8(1), 12703-12703 (2018-08-25)
Hypertensive subjects often exhibit exaggerated cardiovascular reactivity. An overactive orexin system underlies the pathophysiology of hypertension. We examined orexin's roles in eating-associated cardiovascular reactivity in spontaneously hypertensive rats (SHRs) and Wistar-Kyoto (WKY) rats. Results showed eating regular chow or palatable
Ayu Nishii et al.
Frontiers in behavioral neuroscience, 11, 235-235 (2017-12-12)
Increasing clinical evidence suggests that regular physical exercise can prevent or reduce the incidence of stress-related psychiatric disorders including depressive symptoms. Antidepressant effect of regular exercise may be implicated in monoaminergic transmission including serotonergic transmission, activation of the hypothalamic-pituitary-adrenal (HPA)
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