추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
형태
liquid
포함
≤0.1% sodium azide as preservative
종 반응성
human
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
avoid repeated freeze/thaw cycles
배송 상태
wet ice
저장 온도
−20°C
타겟 번역 후 변형
phosphorylation (pSer300)
일반 설명
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300.
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300 in HEK293 cells.
This PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).
면역원
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α
애플리케이션
Immunoblotting (0.5 μg/ml)
Immunocytochemistry (1 μg/ml)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
물리적 형태
In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
재구성
Following initial thaw, aliquot and freeze (-20°C).
분석 메모
Negative Control
HEK293 cells treated with dichloroacetate
HEK293 cells treated with dichloroacetate
Positive Control
HEK293 cells
HEK293 cells
기타 정보
Due to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.
Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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