ABE602

Anti-SATB1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 기술: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• UniProt 수납 번호: Q01826
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SATB1(6304)

설명

일반 설명

Special AT-rich sequence-binding protein 1 (SATB1) contains 2 CUT DNA-binding domains and one homeobox DNA binding domain belonging to the CUT homeobox family. SATB1 contributes to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. SATB1 is organized into a cage-like network anchoring loops of heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex), and transcription factors recruitment. SATB1 is predominantly expressed in thymus. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic.

특이성

This antibody recognizes the C-terminus of SATB1.

면역원

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human SATB1.

애플리케이션

Detect Special AT-rich sequence-binding protein 1 using this rabbit polyclonal antibody, Anti-SATB1 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected SATB1 in human brain tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected SATB1 in human brain cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

품질

Evaluated by Western Blotting in A20 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected SATB1 in 15 µg of A20 cell lysate.

표적 설명

~90 and ~98 kDa observed. Uniprot describes two isoforms at ~86 kDa and ~89 kDa Uncharacterized bands may appear in some lysate(s).

물리적 형태

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
A20 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable