ABE416

Anti-SHMT1 Antibody

from sheep, purified by affinity chromatography

• 동의어(들): Serine hydroxymethyltransferase, cytosolic, SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: sheep
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, mouse
• 기술: ChIP: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_033197
• UniProt 수납 번호: P50431
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SHMT1(6470)

설명

일반 설명

Serine hydroxymethyltransferase (SHMT) is also known as glycine hydroxymethyltransferase or serine methylase, and is an enzyme that catalyzes the reversible conversion of serine and glycine. SHMT is thought to be involved in the epigenetic DNA methylation mechanism and has shown increased activity during cell proliferation and the S phase of the cell cycle. Evidence indicates that SHMT repression is a critical step in the Retinoic Acid-induced cell growth arrest and differentiation pathway.

면역원

KLH-conjugated linear peptide corresponding to mouse SHMT1.

애플리케이션

Anti-SHMT1 Antibody is a highly specific sheep polyclonal antibody, that targets Serine hydroxymethyltransferase & has been tested in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blot Analysis: 2.5 µg/mL of this antibody detected SHMT1 in 10 µg of mouse liver tissue lysate (20 µL of antibody at 5 mg/mL is provided. Please take caution to centrifuge and handle carefully, when pipetting from the tube.).

Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected SHMT1 when coprecipitated with PCNA in pBABE-Puro-SV50-LT transfected HeLa nuclear extracts. MacFarlane A.J., et al. (2011). J Biol Chem. 286(51):44015-22

품질

Evaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 2.5 µg/mL of this antibody detected SHMT1 in 10 µg of human liver tissue lysate (20 µL of antibody at 5 mg/mL is provided. Please take caution to centrifuge and handle carefully, when pipetting from the tube.).

표적 설명

~53 kDa observed. An uncharacterized band may be observed at ~40 kDa in some tissue lysates.

물리적 형태

Affinity purified

Purified sheep polyclonal in buffer containing 0.5M HEPES with 0.05% sodium azide and 50% glycerol.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

분석 메모

Control
Human liver tissue lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1