ABE1859
Anti-KAP-1/TRIM28 Antibody
serum, from rabbit
동의어(들):
Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KAP-1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, RING finger protein 96, Tripartite motif-containing protein 28, KAP-1/TRI
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모든 사진(4)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, human
기술
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
mouse ... Trim28(21849)
일반 설명
Transcription intermediary factor 1-beta (UniProt Q13263; also known as E3 SUMO-protein ligase TRIM28, KAP-1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, Protein phosphatase 1 regulatory subunit 157, RING finger protein 96, TIF1-beta, Transcriptional intermediary factor 1-beta, Tripartite motif-containing 28, Tripartite motif-containing protein 28) is encoded by the TRIM28 (also known as KAP1, PPP1R157, RNF96, TF1B, TIF1B) gene (Gene ID 10155) in human.
Kruppel-associated box-/KRAB-associated protein 1 (KAP-1) is a transcriptional co-repressor that selectively targets the KRAB domain of zinc finger protein-based transcription factors with its N-terminal tripartite RBCC (RING, b-box, coiled-coil) domain. KAP-1 uses its C-terminal PxVxL motif and the PHD-Bromo domain to recruit chromatin modifiers, such as heterochromatin protein 1 (HP-1), Histone-lysine N-methyltransferase SETDB1, nucleosome remodeling and deacetylation (NuRD) complex, and histone deacetylases (HDACs), for transcriptional repression. KAP-1 also plays an essential role in the DNA damage response (DDR). Upon DNA damage, KAP-1 is rapidly phosphorylated by Ataxia telangiectasia mutated (ATM) on Ser824, causing chromatin relaxation to facilitate DNA repair. In addition, SIRT1-mediated KAP-1 deacetylation is reported to stabilize KAP1 and 53BP1 interaction, resulting in an enhancement of NHEJ-mediated repair efficiency in a KAP-1 pSer824-independent manner.
Kruppel-associated box-/KRAB-associated protein 1 (KAP-1) is a transcriptional co-repressor that selectively targets the KRAB domain of zinc finger protein-based transcription factors with its N-terminal tripartite RBCC (RING, b-box, coiled-coil) domain. KAP-1 uses its C-terminal PxVxL motif and the PHD-Bromo domain to recruit chromatin modifiers, such as heterochromatin protein 1 (HP-1), Histone-lysine N-methyltransferase SETDB1, nucleosome remodeling and deacetylation (NuRD) complex, and histone deacetylases (HDACs), for transcriptional repression. KAP-1 also plays an essential role in the DNA damage response (DDR). Upon DNA damage, KAP-1 is rapidly phosphorylated by Ataxia telangiectasia mutated (ATM) on Ser824, causing chromatin relaxation to facilitate DNA repair. In addition, SIRT1-mediated KAP-1 deacetylation is reported to stabilize KAP1 and 53BP1 interaction, resulting in an enhancement of NHEJ-mediated repair efficiency in a KAP-1 pSer824-independent manner.
특이성
Expected to react with both spliced isoforms.
면역원
Epitope: Bromo domain
GST-tagged recombinant protein corresponding to the Bromo domain of human KAP-1/TRIM28.
애플리케이션
Anti-KAP-1/TRIM28 Antibody is an antibody against KAP-1/TRIM28 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
Western Blotting Analysis: A representative lot detected the endogenous KAP-1/Trim28 only in the immunoprecipitats of exogenously expressed FLAG-SirT1 constructs that contain the KAP-1/Trim28-binding domain (a.a. 143-213) using various transfected HEK293T cells (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: A representative lot detected similar level of endogenous KAP-1/Trim28 in HEK293T cells regardless of ionizing radiation treatment or shRNA-mediated SirT1 downregulation (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: A representative lot detected KAP-1/Trim28 in whole cell lysates, but not in BAP1, FoxK1, or FoxK2 immunoprecipitate (Okino, Y., et al. (2015). J. Biol. Chem. 16;290(3):1580-1591).
Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated KAP-1/Trim28 from HEK293T cell lysates (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
Immunocytochemistry Analysis: An 1:1000 dilution from a representative lot detected KAP-1/Trim28 nuclear localization in U2OS cells (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated SirT1 with KAP-1/Trim28 from HEK293T cell lysates (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: An 1:1000 dilution from a representative lot detected KAP-1/Trim28 in mouse embryonic fibroblast (MEF) lysate, as well as downregulated KAP-1/Trim28 in lysates from KAP1 siRNA-treated HEK293T cells (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
Western Blotting Analysis: A representative lot detected similar level of endogenous KAP-1/Trim28 in HEK293T cells regardless of ionizing radiation treatment or shRNA-mediated SirT1 downregulation (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: A representative lot detected KAP-1/Trim28 in whole cell lysates, but not in BAP1, FoxK1, or FoxK2 immunoprecipitate (Okino, Y., et al. (2015). J. Biol. Chem. 16;290(3):1580-1591).
Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated KAP-1/Trim28 from HEK293T cell lysates (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
Immunocytochemistry Analysis: An 1:1000 dilution from a representative lot detected KAP-1/Trim28 nuclear localization in U2OS cells (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated SirT1 with KAP-1/Trim28 from HEK293T cell lysates (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: An 1:1000 dilution from a representative lot detected KAP-1/Trim28 in mouse embryonic fibroblast (MEF) lysate, as well as downregulated KAP-1/Trim28 in lysates from KAP1 siRNA-treated HEK293T cells (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
품질
Evaluated by Western Blotting in HEK293T cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected KAP-1/TRIM28 in 10 µg of HEK293T cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected KAP-1/TRIM28 in 10 µg of HEK293T cell lysate.
표적 설명
~110 kDa observed
물리적 형태
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
Unpurified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Lindsay Kosack et al.
Cancer cell, 35(1), 125-139 (2019-01-16)
The marsupial Tasmanian devil (Sarcophilus harrisii) faces extinction due to transmissible devil facial tumor disease (DFTD). To unveil the molecular underpinnings of this transmissible cancer, we combined pharmacological screens with an integrated systems-biology characterization. Sensitivity to inhibitors of ERBB tyrosine
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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