ABC1435M

Anti-ALIX

from rabbit, purified by affinity chromatography

• 동의어(들): Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, human
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_037506
• UniProt 수납 번호: Q8WUM4
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PDCD6IP(10015)

설명

일반 설명

Programmed cell death 6-interacting protein (UniProt: Q8WUM4; also known as PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95) is encoded by the PDCD6IP (also known as AIP1, ALIX, KIAA1375) gene (gene ID: 10015) in human. ALIX, the mammalian orthologue of xenopus Xp95 and yeast BRO1, is an abundant adaptor protein involved in diverse cellular processes, including actin-based cytoskeleton assembly, integrin-mediated cell adhesion, and ESCRT- (endosomal sorting complex required for transport-) dependent membrane trafficking. ALIX is a Class E VPS protein involved in concentration and sorting of cargo proteins of the multi-vesicular body (MVB) for incorporation into intralumenal vesicles that are generated by invagination and scission from the limiting membrane of the endosome. Sorting of endocytosed membrane proteins by MVBs requires coordinated cargo recognition and assembly of membrane-sculpturing machinery that buds cargo-loaded membrane vesicles into the lumen of endosomes. MVB-mediated sorting is driven by five distinct ESCRT complexes (ESCRT-0, -I, -II and -III, and the Vps4 complex) and associated proteins. ALIX and charged MVB protein 4 (CHMP4), the mammalian orthologue of yeast Snf7, are binding partners involved in a variety of ESCRT-III-mediated membrane-remodeling processes in mammalian cells, including retroviral budding, cytokinetic abscission, biogenesis of exosomes, plasma membrane repair, and ubiquitin-independent MVB sorting of protease-activated receptor 1 (PAR1). In the absence of binding partners, an intramolecular interaction between the Patch 2 region in the Bro1 domain (aa 3-392) and the TSG101-docking site (aa 717-720) in the Pro-rich domain (aa 717-860) locks ALIX in a conformation inaccessible for interaction with CHMP4 and retroviral Gag proteins.

특이성

This rabbit polyclonal antibody detects Programmed cell death 6-interacting protein in human and murine cells.

면역원

Epitope: unknown

His-tagged recombinant fragment corresponding to the first 716 amino acids of human ALIX.

애플리케이션

Anti-ALIX, Cat. No. ABC1435, is a rabbit polyclonal antibody that detects Programmed cell death 6-interacting protein and has been tested ted for use in Immunocytochemistry and Western Blotting.

Research Category
Apoptosis & Cancer

Western Blotting Analysis: A 1:500 dilution from a representative lot detected ALIX in 10 µg of HEK293 cell lysate.

Immunocytochemistry Analysis: A representative lot detected ALIX in Immunocytochemistry applications (Morita, E., et. al. (2007). EMBO J. 26(19):4215-27).

Western Blotting Analysis: A representative lot detected ALIX in Western Blotting applications (Morita, E., et. al. (2007). EMBO J. 26(19):4215-27; Sandrin, V., et. al. (2013). Retrovirology. 10:104; Mercenne, G., et. al. (2015). Elife. 4; Zhai, Q., et. al. (2011). J Virol. 85(1):632-7).

품질

Evaluated by Western Blotting in Hela cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ALIX in 10 µg of HeLa cell lysate.

표적 설명

~90 kDa observed; 96.02 kda calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable