AB951-I

Anti-Neuron Specific Enolase Antibody

from rabbit

• 동의어(들): Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, mouse, rat
• 종 반응성(상동성에 의해 예측): bovine (based on 100% sequence homology)
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI 수납 번호: NP_001966
• UniProt 수납 번호: P09104
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ENO2(2026)

설명

일반 설명

The glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, E.C. 4.2.1.11) is composed of two subunits and exists in three molecular forms in human brain: non-neuronal enolase (NNE; alpha, alpha′-enolase), neuron-specific enolase (NSE; gamma,gamma′-enolase, also known as 14-3-2 protein and NSP) and the short-lived heterodimer alpha, gamma′-enolase. The neuron specific form is a highly acidic homodimeric protin of 78kDa, which has been found in central and peripheral nervous elements and also in many neuroendocrine cells and derivative tumors. NSE is regarded as the best described serum marker for small cell lung cancer. In addition, the measurement of NSE levels in both serum and cerebrospinal fluid following cerebral ischaemia and traumatic head injury provides a reliable laboratory indicator of the degree of brain cell damage and may allow early prediction of prognostic outcome.

특이성

This antibody recognizes a region near the N-terminus of Neuron Specific Enolase.

면역원

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to the near N-terminus of human Neuron Specific Enolase.

애플리케이션

Anti-Neuron Specific Enolase Antibody is a highly specific rabbit polyclonal antibody, that targets Neuron-Specific Enolase & has been tested in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected in Neuron Specific Enolase in human thalamus tissue and glioma sections.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

품질

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Neuron Specific Enolase in 10 µg of rat brain tissue lysate.

표적 설명

~47 kDa observed. Cat. No. AB951, Anti-Neuron Specific Enolase detects Alpha Enolase (ENO1) at ~39kDa However, Neuron Specific Enolase has been observed at ~48 kDa (Braus, B., K., et al (2008). Vet Immunol Immunopathol 124(1-2):177-183.; Feo, S., et al. (2000). FEBS Lett. 473(1):47-52.).

결합

Replaces: AB951

물리적 형태

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Rat brain tissue lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable