AB5252

Anti-Glial Derived Neurotrophic Factor Antibody

serum, Chemicon^®

• 동의어(들): GDNF
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: sheep
• Quality Level: 100
• 항체 형태: serum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, rat, mouse
• 제조업체/상표: Chemicon^®
• 기술: western blot: suitable
• NCBI 수납 번호: NM_000514.2; NM_199231.1; NM_199234.1
• UniProt 수납 번호: P39905
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GDNF(2668)

설명

특이성

Specific for GDNF. No cross reactivity with Neurturin.

면역원

Recombinant human GDNF.

애플리케이션

Anti-Glial Derived Neurotrophic Factor Antibody detects level of Glial Derived Neurotrophic Factor & has been published & validated for use in WB.

Immunoblotting: 1:3,000 using TBS (not PBS) and blocking is best performed with IgG free BSA highest quality. Antibody recognizes ~75 kDa band in non-acid-treated serum samples corresponding to the GDNF/GDNFR complex. When samples are acid treated, a prominent 32 kDa (dimer) and weaker 16 kDa monomer are observed. Acid treatment: homogenized tissue samples in PBS are acid-treated by adding 1N HCL until the pH is less than 3.0 (pH paper strips), incubated 15 minutes at room temperature and then neutralized by adding 1N NaOH to pH 7.6.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

Neuroinflammation & Pain

물리적 형태

Sheep serum. Lyophilized. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue. Contains no preservative. Glycerol (1:1) can be added for additional stability.

저장 및 안정성

Maintain lyophilized material at -20°C to -70°C (dry) for up to twelve months. After reconstitution maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable