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Merck
모든 사진(1)

주요 문서

AB3274

Sigma-Aldrich

Anti-Aquaporin 2 Antibody

Chemicon®, from rabbit

동의어(들):

AQP2, AQP-CD, WCD-CD

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

항체 형태

affinity purified immunoglobulin

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

rat, human

제조업체/상표

Chemicon®

기술

immunohistochemistry: suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... AQP2(359)

특이성

Recognizes Aquaporin 2.

SPECIES REACTIVITIES: The epitope specific for Aquaporin 2 is identical in rat and mouse and only slightly different in human (17/18 amino acids). Reactivity with other species has not yet been tested.

면역원

Highly purified peptide corresponding to residues 254-271 of rat or mouse Aquaporin 2 (SwissProt accession number P34080).

애플리케이션

Anti-Aquaporin 2 Antibody is an antibody against Aquaporin 2 for use in WB, IH.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blotting: 1:200-1:1000 using ECL on rat kidney membranes.

Immunohistochemistry on rat kidney sections.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

물리적 형태

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

저장 및 안정성

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

분석 메모

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 0.5ug of purified peptide with 1 μg of antibody for one hour at room temperature.

Optimal concentrations must be determined by the end user.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


시험 성적서(COA)

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문서 라이브러리 방문

Tanya M Gwathmey et al.
American journal of physiology. Renal physiology, 299(5), F983-F990 (2010-09-03)
The kidney is an important target for the actions of the renin-angiotensin system (RAS) and this tissue contains a complete local RAS that expresses the bioactive peptides angiotensin II (ANG II) and Ang-(1-7). We find both angiotensin type 1 (AT(1)R)
Stine Julie Tingskov et al.
Frontiers in physiology, 10, 948-948 (2019-08-27)
Arginine vasopressin (AVP) mediates water reabsorption in the kidney collecting ducts through regulation of aquaporin-2 (AQP2). Also, estrogen has been known to regulate AQP2. Consistently, we previously demonstrated that tamoxifen (TAM), a selective estrogen receptor modulator, attenuates the downregulation of
Hye-Young Kim et al.
American journal of physiology. Renal physiology, 296(3), F543-F555 (2009-01-09)
Ammonia metabolism and transport are critical for acid-base homeostasis. The ammonia transporter family member Rh C glycoprotein (Rhcg) is expressed in distal renal tubular segments, and its expression is regulated in parallel with renal ammonia metabolism. However, there are inconsistencies
Hyo-Jung Choi et al.
Cells, 9(5) (2020-05-18)
Sorting nexin 27 (SNX27), a PDZ (Postsynaptic density-95/Discs large/Zonula occludens 1) domain-containing protein, cooperates with a retromer complex, which regulates intracellular trafficking and the abundance of membrane proteins. Since the carboxyl terminus of aquaporin-2 (AQP2c) has a class I PDZ-interacting
Emily S Dorey et al.
Physiological reports, 7(21), e14273-e14273 (2019-11-07)
Maternal alcohol consumption can impair renal development and program kidney dysfunction in offspring. Given that most women who drink alcohol cease consumption upon pregnancy recognition, we aimed to investigate the effect of alcohol around the time of conception (PC:EtOH) on

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