AB1876-I

Anti-VEGF-A Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): VEGF-A, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: rat, mouse
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI 수납 번호: NP_001273985
• UniProt 수납 번호: Q00731
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Vegfa(22339) ; rat ... Vegfa(83785)

설명

일반 설명

The human VEGFA, a 45 kDa homodimeric glycoprotein, is one of the most potent angiogenic factors involved in endothelial cell proliferation, migration, and secretion of MMPs. It also serves as an important survival factor for endothelial cells, inhibiting apoptosis. VEGFA belongs to a family that includes VEGFA, VEGFB, VEGFC, VEGFD, and placental growth factor (PLGF). In humans four prominent isoforms of VEGFA are recognized, VEGF121, VEGF165, VEGF189, and VEGF206. In addition, two other less frequent splice variants, VEGF145 and VEGF183, have also been reported. The properties of native VEGF are very similar to VEGF165. Of all the different isoforms of VEGF, the VEGF165 has the highest bioavailability and biological potency. VEGFA action is mediated by VEGF receptor-1 (VEGFR-1) and VEGFR-2. VEGFR-1 (180 kDa, Flt-1) expression is upregulated by hypoxic conditions in the inner core of tumor mass. VEGFR-2 (200 kDa, KDR) is a major mediator of mitogenic and angiogenic permeability enhancing effects of VEGF. It is also reported to mediate survival signals in endothelial cells.

면역원

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to mouse VEGF-A near the N-terminus.

애플리케이션

Anti-VEGF-A Antibody is an antibody against VEGF-A for use in Western Blotting and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected VEGF-A in mouse kidney, mouse cerebellum, mouse spleen, and rat kidney tissue.

Research Category
Signaling

Research Sub Category
Metabolic Hormones & Receptors

품질

Evaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected VEGF-A in 10 µg of mouse lung tissue lysate.

표적 설명

~27 kDa observed

결합

Replaces: AB1876

물리적 형태

Affinity

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable