추천 제품
제품명
Anti-Tyrosine Hydroxylase Antibody, Chemicon®, from sheep
생물학적 소스
sheep
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
rat, mouse
종 반응성(상동성에 의해 예측)
mammals
제조업체/상표
Chemicon®
기술
immunohistochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... TH(7054)
일반 설명
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
특이성
Tyrosine Hydroxylase (TH, Tyrosine Monooxygenase). Cross-reacts with all mammalian forms tested to date and some non-mammalian forms.
면역원
Native tyrosine hydroxylase from rat pheochromocytoma (J. Biol. Chem.,1982, 257:9416-9423).
애플리케이션
Anti-Tyrosine Hydroxylase Antibody is an antibody against Tyrosine Hydroxylase for use in IH & WB.
Immunohistochemistry(peroxidase): A previous lot of this antibody was used at 1:1,000 dilution. The antibody gives specific labeling of noradrenergic axons in primate cerebral cortex (Brain Res., 1989, 500:313-324.).
Note: It is important that you use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.
Optimal working dilutions must be determined by the end user.
Note: It is important that you use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neurotransmitters & Receptors
Neuronal & Glial Markers
품질
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of mouse brain lysates.
표적 설명
~60 kDa
물리적 형태
ImmunoAffinity Purified
Purified sheep polyclonal in buffer containing 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
저장 및 안정성
Stable for 6 months at -20 to -80°C in undiluted aliquots from date of receipt. Store in a NON-self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
분석 메모
Control
Positive: brain (corpus striatum) and adrenal gland. Negative: liver.
Positive: brain (corpus striatum) and adrenal gland. Negative: liver.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
The atypical homeoprotein Pbx1a participates in the axonal pathfinding of mesencephalic dopaminergic neurons.
Sgado, P; Ferretti, E; Grbec, D; Bozzi, Y; Simon, HH
Neural Dev. null
Selective optogenetic activation of rostral ventrolateral medullary catecholaminergic neurons produces cardiorespiratory stimulation in conscious mice.
Abbott, SB; DePuy, SD; Nguyen, T; Coates, MB; Stornetta, RL; Guyenet, PG
The Journal of Neuroscience null
Transsynaptic activity-dependent regulation of axon branching and neurotrophin expression in vivo.
Calinescu, AA; Liu, T; Wang, MM; Borjigin, J
The Journal of Neuroscience null
Genome-wide characterisation of Foxa1 binding sites reveals several mechanisms for regulating neuronal differentiation in midbrain dopamine cells.
Metzakopian, E; Bouhali, K; Alvarez-Saavedra, M; Whitsett, JA; Picketts, DJ; Ang, SL
Development null
Muriel T Davisson et al.
PloS one, 6(12), e29538-e29538 (2012-01-14)
Mutations in the gene encoding the immunoglobulin-superfamily member cell adhesion molecule contactin1 (CNTN1) cause lethal congenital myopathy in human patients and neurodevelopmental phenotypes in knockout mice. Whether the mutant mice provide an accurate model of the human disease is unclear;
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