8.51025

5-Carboxyfluorescein

Novabiochem^®

• 동의어(들): 5-Carboxyfluorescein, 5-FAM
• 실험식(Hill 표기법): C₂₁H₁₂O₇
• CAS Number: 76823-03-5
• Molecular Weight: 376.32
• MDL number: MFCD00036874
• UNSPSC 코드: 12352005
• NACRES: NA.22

속성

• Quality Level: 200
• 제품 라인: Novabiochem^®
• 분석: ≥98% (TLC); ≥99.0% (HPLC)
• 양식: powder
• 반응 적합성: reagent type: fluorescent-labelling reagent
• 제조업체/상표: Novabiochem^®
• 응용 분야: peptide synthesis
• 저장 온도: 2-8°C
• SMILES string: O1C3(c4c(cc(cc4)O)Oc5c3ccc(c5)O)c2c(cc(cc2)C(=O)O)C1=O
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChI key: NJYVEMPWNAYQQN-UHFFFAOYSA-N

설명

일반 설명

This high purity fluorescent dye (λ_ex494 nm, λ_em 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment ^[1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

애플리케이션

5-Carboxyfluorescein Novabiochem^®. CAS 76823-03-5, molar mass 376.32 g/mol.

결합

Replaces: 01-63-0112

분석 메모

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem^® products please click here.

법적 정보

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable