추천 제품
일반 설명
Enrichment of T7 phage displaying target peptides or proteins can easily be accomplished with the T7Select® Biopanning Kit. The kit contains reagents to perform biopanning with target ligands using 96-well microtiter plates or similar solid phases. Biopanning procedures can be tested and optimized using the positive and negative controls provided in the kit. The supplied Positive Control Lysate contains a T7Select415-1 recombinant that displays the S•Tag peptide. When combined with the Negative Control Lysate at known ratios, the degree of enrichment of high affinity binding phage can be monitored by a simple plaque lift assay with the provided detection reagents.
Biopanning selection
Biopanning requires both binding and elution conditions that maintain the infectivity of the phage. The stability of the T7 phage particle enables the use of a variety of elution conditions during biopanning. Phage maintain infectivity following treatment with 1% SDS, 5 M NaCl, 4 M urea, 2 M guanidine-HCl, 10 mM EDTA, reducing conditions (up to 100 mM DTT), and a pH range of 4 to 10. The wide range of conditions available for T7Select biopanning expands the range of targets that can be used successfully.
Biopanning selection
Biopanning requires both binding and elution conditions that maintain the infectivity of the phage. The stability of the T7 phage particle enables the use of a variety of elution conditions during biopanning. Phage maintain infectivity following treatment with 1% SDS, 5 M NaCl, 4 M urea, 2 M guanidine-HCl, 10 mM EDTA, reducing conditions (up to 100 mM DTT), and a pH range of 4 to 10. The wide range of conditions available for T7Select biopanning expands the range of targets that can be used successfully.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
성분
- 25 g Blocking Reagent
- 2 x 50 ml 10X TBST Wash Buffer
- 10 ml T7 Elution Buffer
- 0.2 ml BL21 Glycerol Stock
- 0.2 ml BLT5403 Glycerol Stock
- 0.2 ml BLT5615 Glycerol Stock
- 1010 pfu T7Select Positive Control Lysate
- 1010 pfu T7Select Negative Control Lysate
- 50 μl S-protein HRP Conjugate
- 2 ml T7Select S-protein
- 50 ml SuperSignal Substrate
- pkg/25 g LOCATOR Luminescent Labels
- pkg/25 Development Folders
경고
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
법적 정보
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
T7SELECT is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
관련 콘텐츠
Major technological advances have made the production of monoclonal antibodies quicker and more efficient. There are three established platforms for antibody discovery. We offer reagents for production of monoclonal antibody libraries using each of these techniques.
최근 기술 분야에서 중대한 발전들이 이뤄지면서 단클론 항체 생성의 속도와 효율성이 더욱 높아졌습니다. 항체 발견을 위해 확립된 플랫폼은 3가지가 있습니다. Merck는 이 기법들을 각각 이용해서 단클론 항체 라이브러리를 생성하는 데 필요한 시약을 제공합니다.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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