6502

Trypsin, Bovine Pancreas

• 동의어(들): Trypsin, Bovine Pancreas
• CAS Number: 9002-07-7
• 효소 위원회 번호: 3.4.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00040513
• UNSPSC 코드: 12352202
• NACRES: NA.25

속성

• 양식: solid
• Quality Level: 100
• 특이 활성도: ≥2,500 USP units/mg dry wt
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated (hygroscopic)
• solubility: HCl: 1 mM; neutral buffer: soluble; water: soluble
• 외래 활성: Chymotrypsin ≤50 NF units/mg dry wt.
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides and proteins at the C-terminal side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Note: 1 MU = 1,000,000 units.

Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Has an optimal pH of 8.5-8.8.

Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units.

경고

Toxicity: Irritant (B)

단위 정의

One USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.

물리적 형태

Salt-free

재구성

Following reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.

기타 정보

Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3^rd ed. Vol. 3, 17.
Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem.239, 1799.
Hummel, B.C.W. 1955. Can. J. Biochem. Physiol.37, 1393.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1