616407

TACE Substrate IV, Fluorogenic

• 동의어(들): TACE Substrate IV, Fluorogenic, TNF-α Convertase Enzyme Substrate IV, Fluorogenic, Abz-LAQAVRSSSR-Dpa, ADAM-17 Substrate IV, Fluorogenic
• 실험식(Hill 표기법): C₅₉H₉₃N₂₃O₂₀
• Molecular Weight: 1444.51
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• 분석: ≥95% (HPLC)
• Quality Level: 100
• 양식: lyophilized solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated; protect from light
• 색상: yellow
• solubility: DMSO: 5 mg/mL
• 형광: λ_ex ~320 nm; λ_em ~420 nm
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

An internally quenched, fluorogenic, 10-amino acid peptide substrate that is useful for monitoring TNF-α Convertase Enzyme (TACE) activity by a continuous fluorometric assay. Also useful for screening TACE inhibitors (k_cat = 21.6 s^-1 when the substrate concentration is half the V_max, S_0.5 = 342 µM). The substrate is cleaved at the Ala-Val amide bond. The substrate is not substantially cleaved by MMP-1, MMP-9, or MMP-13. Displays improved solubility in aqueous buffers. Excitation max: ~320 nm; Emission max: ~420 nm.

An internally-quenched fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity and high-throughput screening of TACE inhibitors (k_cat = 21.6 s^-1 and substrate concentration at half the V_max, S_0.5 = 342 µM). The substrate is cleaved at Ala-Val amide bond. Acts as a poor substrate for MMP-1, MMP-9, and MMP-13. Displays better solubility in aqueous buffers.

생화학적/생리학적 작용

Cell permeable: no

Primary Target
Fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity

Product does not compete with ATP.

Reversible: no

kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM

경고

Toxicity: Standard Handling (A)

서열

Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa

물리적 형태

Supplied as a trifluoroacetate salt.

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

기타 정보

Jin, G., et al. 2002. Anal. Biochem.302, 269.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable