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Merck
모든 사진(1)

문서

494460

Millipore

Octyl β-D-glucopyranoside

ULTROL® Grade, ≥99% (TLC), non-ionic detergent to solubilize membrane-bound proteins in their native state

동의어(들):

n-Octyl-β-D-glucopyranoside, ULTROL® Grade, OG, n-Octylglucoside

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About This Item

실험식(Hill 표기법):
C14H28O6
CAS Number:
Molecular Weight:
292.37
MDL number:
UNSPSC 코드:
12161902
NACRES:
NA.21

Quality Level

분석

≥99% (TLC)

형태

solid

제조업체/상표

Calbiochem®

저장 조건

OK to freeze
desiccated (hygroscopic)

응집 번호

84

불순물

≤0.05% n-octanol

색상

white

CMC

20 - 25 mM

solubility

water: 100 mg/mL

배송 상태

ambient

저장 온도

15-25°C

InChI

1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1

InChI key

HEGSGKPQLMEBJL-RKQHYHRCSA-N

일반 설명

A highly purified nonionic detergent intended for solubilizing membrane-bound proteins in their native state, and for the preparation of lipid vesicles. Its well-defined chemical structure, small uniform micelles, and high water solubility make it superior to most other nonionic detergents for membrane solubilization. Because of its high critical micelle concentration (20-15 mM), it has the additional advantage of being removed easily by dialysis when compared to bile salts. Aggregation number: 84.
Absorbance (10%, H2O, 260 nm):≤0.3; conductivity (100 mM, H2O):≤100 µmhos.
Highly purified non-ionic detergent intended for solubilizing membrane-bound proteins in their native state. Aggregation number: 84.

경고

Toxicity: Standard Handling (A)

재구성

Following reconstitution, store in the refrigerator (4°C) under sterile conditions. Aqueous stock solutions are stable for 3 months at 4°C. Solutions are susceptible to hydrolysis (especially in strong acid) and enzymatic degradation.

기타 정보

Hoshino, T., et al. 1992. J. Biol. Chem.267, 21313.
Levy, D., et al. 1992. Biochim. Biophys. Acta1107, 283.
Nunn, R.S., et al. 1992. J. Mol. Biol.228, 1259.
Lorber, B., et al. 1990. Biochim. Biophys. Acta1023, 254.
Jopski, B., et al. 1989. Biochim. Biophys. Acta978, 79.
Camm, E.L., et al. 1981. Plant Physiol.67, 1061.
Gould, R.J., et al. 1981. Biochemistry20, 6776.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

B Jopski et al.
Biochimica et biophysica acta, 978(1), 79-84 (1989-01-16)
Hemoglobin (Hb) was encapsulated in phosphatidylcholine vesicles by removal of the detergent n-octyl beta-D-glucoside (OG) or n-octyltetraoxyethylene (C8E4) out of mixed detergent-lipid micelles in Hb solution. Three types of apparatus were used for dialysis. Dialysis buffer flow rates, the surface
B Lorber et al.
Biochimica et biophysica acta, 1023(2), 254-265 (1990-04-13)
The commercial non-ionic detergent octyl beta-D-glucopyranoside is often contaminated by significant amounts of UV absorbing and/or ionic compounds that can associate with membrane proteins. Such impurities can be monitored by several techniques (i.e., spectrophotometry, size exclusion chromatography, and pH, conductivity
R S Nunn et al.
Journal of molecular biology, 228(4), 1259-1262 (1992-12-20)
The LH1 light harvesting complex has been purified from a mutant of the photosynthetic bacterium Rhodobacter sphaeroides which synthesizes LH1 as the sole pigment protein. Crystallization trials using polyethylene glycol as the precipitant in the presence of the detergent n-octyl
D Lévy et al.
Biochimica et biophysica acta, 1107(2), 283-298 (1992-06-30)
The Ca(2+)-ATPase from skeletal muscle sarcoplasmic reticulum was reconstituted into sealed phospholipid vesicles using the method recently developed for bacteriorhodopsin (Rigaud, J.L., Paternostre, M.T. and Bluzat, A. (1988) Biochemistry 27, 2677-2688). Liposomes prepared by reverse-phase evaporation were treated with various
E L Camm et al.
Plant physiology, 67(5), 1061-1063 (1981-05-01)
The use of the non-ionic detergent octyl beta-d-glucoside has allowed the demonstration in spinach of a chlorophyll a+b-protein complex of apparent molecular weight 29 kilodaltons (Camm and Green, 1980, Plant Physiol 66: 428-432). Complexes analogous to this one also can

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