48-610MAG

MILLIPLEX MAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay

• 동의어(들): Magnetic Bead STAT Kit, STAT Cell Signaling Kit
• UNSPSC 코드: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

속성

• 종 반응성: human
• Quality Level: 200
• 제조업체/상표: Milliplex^®
• 기술: multiplexing: suitable
• 검출 방법: fluorometric (Luminex xMAP)
• 저장 온도: 2-8°C

설명

일반 설명

The STAT (Signal Transducers and Activators of Transcription) family, consisting of seven mammalian members, regulates many aspects of growth, survival and differentiation in cells. The extracellular binding of cytokines, hormones, growth factors and neurotransmitters to receptors induces phosphorylation of the intracellular Janus kinases (JAKs) that, in turn, phosphorylate STAT proteins at conserved tyrosine residues, activating SH2-mediated dimerization followed by translocation to the nucleus. STAT dimers then bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes.

특이성

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

애플리케이션

• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 μL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).

Research Category
Signaling|Apoptosis & Cancer

The analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).

Used to detect/quantify:STAT1 (Tyr701)|STAT2 (Tyr690)|STAT3 (Tyr705)|STAT5A/B (Tyr694/699)|STAT6 (Tyr641)

포장

96-well plate

저장 및 안정성

Recommended storage for kit components is 2 - 8°C.

법적 정보

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

안전성 정보

• 픽토그램: GHS05,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H318,H410
• 예방조치 성명서: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable