480401-M

NFAT Inhibitor, Cell-Permeable

The NFAT Inhibitor, Cell-Permeable controls the biological activity of NFAT. This small molecule/inhibitor is primarily used for Inflammation/Immunology applications.

• 동의어(들): NFAT Inhibitor, Cell-Permeable, 11R-VIVIT, Nuclear Factor of Activated T cells Inhibitor, RRRRRRRRRRR-GGG- MAGPHPVIVITGPHEE, 11R-VIVIT, Nuclear Factor of Activated T cells Inhibitor, RRRRRRRRRRR-GGG-MAGPHPVIVITGPHEE
• 실험식(Hill 표기법): C₁₄₇H₂₅₉N₆₇O₃₆S
• Molecular Weight: 3573.12
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: ≥95% (HPLC)
• 양식: lyophilized solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated (hygroscopic); protect from light
• 색상: white
• solubility: water: 1 mg/mL; DMSO: 5 mg/mL
• 저장 온도: −20°C

설명

일반 설명

A cell-permeable version of NFAT Inhibitor (Cat. No. 480402) that is modified at the N-terminal with an eleven arginine transduction domain and a three glycine linker sequence. Prevents the activation and proliferation of T cells both in vitro ( ~ 43% at 1 µM using mixed lymphocyte cultures) and in vivo (10 mg/kg using C3H/HeN mice). Shown to be effective in providing immunosuppression in a murine islet xenograft model. Unlike Cyclosporin A (Cat. No. 239835) and FK506, 11R-VIVIT does not cause side effects outside the immune system as a result of calcineurin (Cat. No. 539568) inhibition and therefore serves as a less toxic alternative for in vivo applications. The functional half-life of 11R-VIVIT has been estimated to be ~30 h in Jurkat cells.

애플리케이션

Use at 1 µM for preventing the activation and proliferation of T cells

생화학적/생리학적 작용

Primary Target
NFAT

경고

Toxicity: Harmful (C)

서열

H-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Gly-Met-Ala-Gly-Pro-His-Pro-Val-Ile-Val-Ile-Thr-Gly-Pro-His-Glu-Glu-OH

물리적 형태

Supplied as a trifluoroacetate salt.

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

기타 정보

Noguchi, H., et al. 2004. Nat. Med.10, 305.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable