454578

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL)

lyophilized, clone R1JHL, Calbiochem^®

• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: R1JHL, monoclonal
• 양식: lyophilized
• 미포함: preservative
• 종 반응성: rat
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 동형: IgG
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Mouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.

Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.

This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.

면역원

Rat

a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1

애플리케이션

Immunoblotting (1:300, colorimetric)

Immunoprecipitation (3 µg/200 µg lysate)

경고

Toxicity: Standard Handling (A)

물리적 형태

Lyophilized from cell culture supernatant.

재구성

Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

분석 메모

Positive Control
Rat cortex tissue extracts

기타 정보

Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.

Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci.14, 3180
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Science256, 1217.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable