407252

Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli

• 동의어(들): mIGF-I
• UNSPSC 코드: 12352202

속성

• 분석: ≥98% (SDS-PAGE and HPLC)
• Quality Level: 100
• 양식: lyophilized
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 불순물: ≤1 ng/μg Endotoxin (ng/μg IGF-I)
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Full-length, recombinant, mouse insulin-like growth factor I (IGF-I) expressed in E. coli. IGF-I regulates proliferation and survival signals through the Akt pathway. Mouse IGF-I shares 94% sequence identity with human IGF-I and exhibits cross-species reactivity. Useful in the study of cell proliferation and survival signals.

생화학적/생리학적 작용

ED₅₀: ≤2 ng/ml (corresponding to a specific activity of ≥1 x 10⁶ units/mg) as determined by a cell proliferation assay using FDC-P1 cells. For most in vitro applications IGF-I exerts is biological activity in the concentration range of 1-20 ng/ml.

경고

Toxicity: Standard Handling (A)

물리적 형태

Sterile-filtered prior to lyophilization. Contains no additives.

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions. Aliquots may be stored at 4°C for up to 1 week without loss of biological activity.

Reconstitute with sterile H₂O to a final concentration of 0.1-1 mg/ml. Further dilute with aqueous buffer before use.

기타 정보

Clemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
Bell, G.I., et al. 1986. Nucleic Acids Res.14, 7873.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable