375228

Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda

• 동의어(들): rhHGF
• UNSPSC 코드: 12352202

속성

• 분석: ≥95% (SDS-PAGE)
• Quality Level: 100
• 양식: lyophilized
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 불순물: ≤1 EU/μg endotoxin (EU/μg HGF)
• 배송 상태: ambient
• 저장 온도: −70°C

설명

일반 설명

Recombinant, human hepatocyte growth factor expressed in Spodoptera frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.

생화학적/생리학적 작용

ED₅₀ = 20-40 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in an HGF-responsive epithelial cell line, 4MBr-5

경고

Toxicity: Standard Handling (A)

물리적 형태

Lyophilized from a sterile-filtered solution in 20 mM Sodium Phosphate, 0.5 M NaCl, 50 µg BSA/µg HGF, pH 7.0.

재구성

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥1 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.

기타 정보

Kaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3