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Merck
모든 사진(1)

주요 문서

371723

Sigma-Aldrich

Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC 코드:
12352203
가격 및 재고 정보를 현재 이용할 수 없음

생물학적 소스

rabbit

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

polyclonal

양식

liquid

미포함

preservative

종 반응성(상동성에 의해 예측)

mammals

제조업체/상표

Calbiochem®

저장 조건

OK to freeze
avoid repeated freeze/thaw cycles

동형

IgG

배송 상태

wet ice

저장 온도

−70°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... GNAI1(2770)

일반 설명

Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355), rabbit polyclonal, recognizes Giα-1 and Giα-2 subunits. It is validated for use in Western blotting.
Protein A purified rabbit polyclonal antibody. Recognizes Giα-1 and Giα-2 subunit proteins.
Recognizes Giα-1 and Giα-2 subunits. Does not cross-react with G3α-3 or Goα subunits.

면역원

a synthetic peptide [(C)KNNLKDCGLF] corresponding to amino acids at the C-terminus of mammalian Giα-1 and Giα-2, conjugated to KLH

애플리케이션

Immunoblotting (1:1000)

경고

Toxicity: Standard Handling (A)

물리적 형태

In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Does not cross-react with Giα-3 and Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Y Xu et al.
Infection and immunity, 64(2), 593-599 (1996-02-01)
In previous studies, an in vitro ADP-ribosylation assay was developed to quantitatively evaluate the in vivo ADP-ribosylation of eukaryotic target proteins in intact Chinese hamster ovary (CHO) cells by pertussis toxin (PT). Immunoblot analysis identified the two PT-sensitive target proteins
C Puma et al.
Journal of neurochemistry, 78(5), 960-971 (2001-09-13)
The chemokine IL-8 is known to be synthesized by glial cells in the brain. It has traditionally been shown to have an important role in neuroinflammation but recent evidence indicates that it may also be involved in rapid signaling in
K Takano et al.
Endocrinology, 138(6), 2405-2409 (1997-06-01)
SRIF activates an inwardly rectifying K+ current in human GH-secreting adenoma cells. Activation of this K+ current induces hyperpolarization of the membrane and abolishment of action potential firing. This mechanism is an essential mechanism for SRIF-induced decrease in intracellular Ca2+
Jennifer S Ignatious Raja et al.
The European journal of neuroscience, 39(8), 1245-1255 (2014-01-22)
Intracellular signaling in insect olfactory receptor neurons remains unclear, with both metabotropic and ionotropic components being discussed. Here, we investigated the role of heterotrimeric Go and Gi proteins using a combined behavioral, in vivo and in vitro approach. Specifically, we
Suzy D Carvalho-Bianco et al.
Molecular endocrinology (Baltimore, Md.), 18(7), 1840-1849 (2004-05-08)
Whereas many cardiac symptoms of thyrotoxicosis resemble those of the hyperadrenergic state, circulating catecholamines are reduced or normal in this condition. To test the hypothesis that the thyrotoxic heart is hypersensitive to catechol-amines, we studied beta-adrenergic signaling in a transgenic

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