Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355), rabbit polyclonal, recognizes Giα-1 and Giα-2 subunits. It is validated for use in Western blotting.
Protein A purified rabbit polyclonal antibody. Recognizes Giα-1 and Giα-2 subunit proteins.
Recognizes Giα-1 and Giα-2 subunits. Does not cross-react with G3α-3 or Goα subunits.
면역원
a synthetic peptide [(C)KNNLKDCGLF] corresponding to amino acids at the C-terminus of mammalian Giα-1 and Giα-2, conjugated to KLH
애플리케이션
Immunoblotting (1:1000)
경고
Toxicity: Standard Handling (A)
물리적 형태
In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
재구성
Following initial thaw, aliquot and freeze (-70°C).
기타 정보
Does not cross-react with Giα-3 and Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215. Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Infection and immunity, 64(2), 593-599 (1996-02-01)
In previous studies, an in vitro ADP-ribosylation assay was developed to quantitatively evaluate the in vivo ADP-ribosylation of eukaryotic target proteins in intact Chinese hamster ovary (CHO) cells by pertussis toxin (PT). Immunoblot analysis identified the two PT-sensitive target proteins
Journal of neurochemistry, 78(5), 960-971 (2001-09-13)
The chemokine IL-8 is known to be synthesized by glial cells in the brain. It has traditionally been shown to have an important role in neuroinflammation but recent evidence indicates that it may also be involved in rapid signaling in
SRIF activates an inwardly rectifying K+ current in human GH-secreting adenoma cells. Activation of this K+ current induces hyperpolarization of the membrane and abolishment of action potential firing. This mechanism is an essential mechanism for SRIF-induced decrease in intracellular Ca2+
The European journal of neuroscience, 39(8), 1245-1255 (2014-01-22)
Intracellular signaling in insect olfactory receptor neurons remains unclear, with both metabotropic and ionotropic components being discussed. Here, we investigated the role of heterotrimeric Go and Gi proteins using a combined behavioral, in vivo and in vitro approach. Specifically, we
Whereas many cardiac symptoms of thyrotoxicosis resemble those of the hyperadrenergic state, circulating catecholamines are reduced or normal in this condition. To test the hypothesis that the thyrotoxic heart is hypersensitive to catechol-amines, we studied beta-adrenergic signaling in a transgenic