251515-M

DAF-FM

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• 동의어(들): DAF-FM, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein
• 실험식(Hill 표기법): C₂₁H₁₄F₂N₂O₅
• CAS Number: 254109-20-1
• Molecular Weight: 412.34
• UNSPSC 코드: 12352200

속성

• Quality Level: 100
• 분석: ≥98% (HPLC)
• 양식: solution
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; protect from light
• 색상: pale yellow
• 형광: λ_ex ~500 nm; λ_em ~515 nm
• 저장 온도: −20°C
• InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
• InChI key: DIJCILWNOLHJCG-UHFFFAOYSA-N

설명

일반 설명

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

생화학적/생리학적 작용

Primary Target
Nitric oxide (NO) fluorescent indicator

경고

Toxicity: Irritant (B)

물리적 형태

In DMSO.

재구성

Following initial thaw, aliquot and freeze at -20°C. Use immediately after dilution, discard any unused portion.

기타 정보

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew. Chem. Int. Ed.38, 3209.

법적 정보

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): 188.6 °F
• Flash Point (°C): 87 °C