추천 제품
생물학적 소스
mouse
Quality Level
결합
biotin conjugate
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
4G10®, monoclonal
종 반응성
human
종 반응성(상동성에 의해 예측)
all
제조업체/상표
Upstate®
기술
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG2bκ
배송 상태
wet ice
타겟 번역 후 변형
phosphorylation (pTyr)
유전자 정보
human ... PID1(55022)
일반 설명
Anti-phosphotyrosine monoclonal antibody, clone 4G10 (cat.# 05-321), cross-linked to biotin.
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.
Description
Anti-phosphotyrosine monoclonal antibody, clone 4G10 (cat. # 05-321), cross-linked to biotin.
Description
Anti-phosphotyrosine monoclonal antibody, clone 4G10 (cat. # 05-321), cross-linked to biotin.
특이성
Expected to cross-react with rat and mouse.
Recognizes tyrosine-phosphorylated proteins from all species.
면역원
Phosphotyramine-KLH.
애플리케이션
Anti-Phosphotyrosine Antibody, clone 4G10, Biotin Conjugate detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is published in peer reviewed journals and validated for use in IC, IP, WB.
Immunoprecipitation:
4 μg of a previous lot, used in conjunction with Streptavidin, agarose conjugate (Catalog # 16-126), immunoprecipitated phosphotyrosine containing proteins from a lysate of EGFstimulated A431 cells.
Note: To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
Immunocytochemistry:
5-10 μg/mL of a previous lot gave positive immunostaining of EGF-stimulated A431 cells that had been fixed with ethanol:acetic acid [1:1].
Application Notes
For use in applications in which a biotin conjugate is advantageous, such as WB and IC.
4 μg of a previous lot, used in conjunction with Streptavidin, agarose conjugate (Catalog # 16-126), immunoprecipitated phosphotyrosine containing proteins from a lysate of EGFstimulated A431 cells.
Note: To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
Immunocytochemistry:
5-10 μg/mL of a previous lot gave positive immunostaining of EGF-stimulated A431 cells that had been fixed with ethanol:acetic acid [1:1].
Application Notes
For use in applications in which a biotin conjugate is advantageous, such as WB and IC.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
품질
Routinely evaluated by Western Blot on EGF treated A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Tyrosine phosphorylated proteins on 10 μg of EGF treated A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Tyrosine phosphorylated proteins on 10 μg of EGF treated A431 lysates.
표적 설명
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
물리적 형태
PBS containing 0.05% sodium azide.
Protein G Purified
저장 및 안정성
9 months at 2-8°C from date of shipment. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
분석 메모
Control
Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells
Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Occurrence and phase distribution of selected pharmaceuticals in the Yangtze Estuary and its coastal zone.
Yang Y, Fu J, Peng H, Hou L, Liu M, Zhou JL
Journal of Hazardous Materials null
Christopher N Davis et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(8), 2953-2958 (2006-02-16)
Interleukin (IL)-1beta is a pluripotent proinflammatory cytokine that signals through the type-I IL-1 receptor (IL-1RI), a member of the Toll-like receptor family. In hypothalamic neurons, binding of IL-1beta to IL-1RI mediates transcription-dependent changes that depend on the recruitment of the
W H Ho et al.
The Journal of biological chemistry, 270(24), 14523-14532 (1995-06-16)
The heregulin family of polypeptides arise as splice variants from a single gene and share a conserved epidermal growth factor (EGF)-like domain thought to be the major determinant of their biological activities. We report here the cloning of a novel
H Nishizumi et al.
The Journal of experimental medicine, 187(8), 1343-1348 (1998-05-23)
B cells from young lyn-/- mice are hyperresponsive to anti-IgM-induced proliferation, suggesting involvement of Lyn in negative regulation of B cell antigen receptor (BCR)-mediated signaling. Here we show that tyrosine phosphorylation of FcgammaRIIB and CD22 coreceptors, which are important for
Sandra Odom et al.
The Journal of experimental medicine, 199(11), 1491-1502 (2004-06-03)
A role for Lyn kinase as a positive regulator of immunoglobulin (Ig)E-dependent allergy has long been accepted. Contrary to this belief, Lyn kinase was found to have an important role as a negative regulator of the allergic response. This became
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