추천 제품
생물학적 소스
human
Quality Level
재조합
expressed in baculovirus infected Sf21 cells
분자량
Mw 77 kDa
제조업체/상표
Upstate®
기술
activity assay: suitable (kinase)
NCBI 수납 번호
UniProt 수납 번호
유전자 정보
human ... PIP5K1C(23396)
일반 설명
N-terminal 6His-tagged, recombinant human PIP5K1γ, full length.
Product Source: Expressed by baculovirus in Sf21 insect cells
애플리케이션
Active, N-terminal 6His-tagged, recombinant human PIP5K1γ, full length. For use in Kinase Assays.
Research Category
Signaling
Signaling
Research Sub Category
Oncology
Inflammation & Autoimmune Mechanisms
Diabetes
Alzheimer′s Disease
Neurodegenerative Diseases
Oncology
Inflammation & Autoimmune Mechanisms
Diabetes
Alzheimer′s Disease
Neurodegenerative Diseases
생화학적/생리학적 작용
Protein Target: PIP5K1γ
Target Sub-Family: PIPK
포장
Also available in 250μg size as catalog # 14-845M - Call for pricing and availability.
품질
Routinely evaluated in the PIProfiler.
물리적 형태
10µg of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Immobilized metal affinity chromatography (IMAC)
저장 및 안정성
6 months at -70°C
기타 정보
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Development of a High-Throughput Screening Assay to Identify Inhibitors of the Lipid Kinase PIP5K1C.
Brittany D Wright et al.
Journal of biomolecular screening, 20(5), 655-662 (2014-12-24)
Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) regulate a variety of cellular processes, including signaling through G protein-coupled receptors (GPCRs), endocytosis, exocytosis, and cell migration. These lipid kinases synthesize phosphatidylinositol 4,5-bisphosphate (PIP2) from phosphatidylinositol 4-phosphate [PI(4)P]. Because small-molecule inhibitors of these lipid kinases
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