1.32647

Alcian blue staining kit, pH 1.0

for the detection of strongly sulfated mucins for microscopy

속성

• Quality Level: 400
• IVD: for in vitro diagnostic use
• pH: 1.0
• 응용 분야: clinical testing; diagnostic assay manufacturing; hematology; histology
• 저장 온도: 15-25°C

설명

일반 설명

The ready-to-use Alcian blue stainig kit, pH 1.0 - for the detection of strongly sulfated mucins - for microscopy, can be used for this staining in histological tissue. Alcian blue pH 1.0 is used to stain strongly sulfated mucins in human-histological specimen materials, for example histological sections of e. g. colon or bronchus. The dye Alcian blue 8 GX (Product number 1.01647) is a water-soluble phthalocyanine dye. Acidic mucins have a negative charge and bind the positively charged Alcian blue 8 GX staining dye.
A solution of hydrochloric acid and Alcian blue is used for the staining procedure. At a strongly acidic pH of 1.0, this solution suppresses the dissociation of the carboxyl groups and selectively stains only the strongly sulfated mucins and polyanions. Counterstaining takes place with a nuclear fast red/aluminium sulfate solution. The undifferentiated method (Alcian blue 8 GX solution, pH 2.5) enables only the detection of acidic glycoproteins, e. g. mucins, without distinguishing between carboxyl and sulfate groups.
The package is sufficient for approx. 2500 applications. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.

애플리케이션

For the detection of strongly sulfated mucins.

분석 메모

Nuclei: red
Strongly sulfated mucins: light blue

안전성 정보

• 픽토그램: GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H290 - H318
• 예방조치 성명서: P234 - P280 - P305 + P351 + P338 - P390
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1
• Storage Class Code: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 2