추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
rat, human
종 반응성(상동성에 의해 예측)
mouse (based on 100% sequence homology)
기술
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
unmodified
유전자 정보
mouse ... Pick1(18693)
일반 설명
PRKCA-binding protein (UniProt Q62083; also known as Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein) is encoded by the Pick1 (also known as Prkcabp) gene (Gene ID 18693) in murine species. PICK1 is a scaffolding protein expressed in many tissues, where it regulates trafficking of multiple membrane proteins. The PDZ (PSD-95/Dlg/ZO-1/DHR (Dlg homologous region) domain (a.a. 22-105) mediates PICK1 interaction with proteins containing PDZ-binding motifs, such as PKC and acid sensing ion channels (ASICs), while the lipid-binding AH (arfaptin homology)/BAR (Bin/amphiphysin/Rvs) domain (a.a. 144-357) mediates PICK1 homo-dimerization and membrane localization. Pick1 gene-disruption results in downregulated ASICs current and calcium transients during process of ischemia-reperfusion and protects against primary murine cortical neurons against injury caused by OGD-Rep (oxygen and glucose deprivation followed by reperfusion). PICK1 is also shown to act as a negatively regulator against ASIC1 phosphorylation/activation by PKA in rat pulmonary arterial smooth muscle cells, where PICK1 facilitates calcineurin-mediated ASIC1 dephosphorylation.
특이성
This rabbit polyclonal antibody targets the C-terminal region of mouse and rat PICK1, as well as human PICK1 spliced isoform 1, but not isoform 2, reported by UniProt (Q62083, Q9EP80, Q9NRD5).
면역원
Epitope: C-terminus
GST-tagged recombinant mouse PICK1 C-terminal fragment.
애플리케이션
Anti-PICK1, Cat. No. 07-293-I, is a highly specific rabbit polyclonal antibody that targets PRKCA-binding protein and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PICK1 in human cerebellum, kidney, and pancreas tissue sections.
Research Category
Neuroscience
Neuroscience
품질
Evaluated by Western Blotting in PC12 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PICK1 in 10 µg of PC12 rat pheochromocytoma cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PICK1 in 10 µg of PC12 rat pheochromocytoma cell lysate.
표적 설명
~55 kDa observed. 46.60 kDa (human/mouse) and 46.61 kDa (rat) calculated. The larger-than-calculated band size is consistent with that reported in the literature (Xia, J., et al. (1999). Neuron. 22(1):179 187).
물리적 형태
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 제품 선택기 도구.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.